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Browsing by Author "Mhlanga, Musa"

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Chromosomal contact permits transcription between coregulated genes

Author: Fanucchi, Stephanie ; Shibayama, Y ; Burd, S ; Weinberg, MS ; Mhlanga, Musa Date: Oct 2013 Transcription of coregulated genes occurs in the context of long-range chromosomal contacts that form multigene complexes. Such contacts and transcription are lost in knockout studies of transcription factors and structural chromatin proteins. ... Read more
The emerging molecular biology toolbox for the study of long noncoding RNA biology

Author: Fok, Ezio T ; Scholefield, Janine ; Fanucchi, Stephanie ; Mhlanga, Musa Date: Oct 2017 Long noncoding RNAs (lncRNAs) have been implicated in many biological processes. However, due to the unique nature of lncRNAs and the consequential difficulties associated with their characterization, there is a growing disparity between the ... Read more
Enhancer-derived lncRNAs regulate genome architecture: fact or fiction?

Author: Fanucchi, Stephanie ; Mhlanga, Musa Date: Jun 2017 How does the non-coding portion of the genome contribute to the regulation of genome architecture? A recent paper by Tan et al. focuses on the relationship between cis-acting complex-trait-associated lincRNAs and the formation of chromosomal ... Read more
High-throughput SNP genotyping: combining tag SNPs and molecular beacons

Author: Barreiro, LB ; Henriques, S ; Mhlanga, Musa Date: Oct 2009 In the last decade, molecular beacons have emerged to become a widely used tool in the multiplex typing of single nucleotide polymorphisms (SNPs). Improvements in detection technologies in instrumentation and chemistries to label these probes ... Read more
Host gene targets for novel influenza therapies elucidated by high-throughput RNA interference screens

Author: Meliopoulos, VA ; Andersen, LE ; Birrer, KF ; Simpson, KJ ; Lowenthal, JW ; Bean, AGD ; Stambas, J ; Stewart, CR ; Tompkins, SM ; Van Beusechem, VW ; Fraser, I ; Mhlanga, Musa ; Barichievy, S ; Smith, Q ; Leake, D ; Karpilow, J ; Buck, A ; Jona, G ; Tripp, RA Date: Jan 2012 Influenza virus encodes only 11 viral proteins but replicates in a broad range of avian and mammalian species by exploiting host cell functions. Genome-wide RNA interference (RNAi) has proven to be a powerful tool for identifying the host ... Read more
International Genetically Engineered Machine (iGEM) Competition

Author: Sparrow, RW ; Madigoe, E ; Martens, D ; Mhlanga, Musa ; Kowalenko, R ; Rubin, D ; Rumbold, K ; Sherwell, D ; Weinberg, M Date: Jul 2010 iGEM, the International Genetically Engineered Machine competition, is an initiative from MIT and has become the premiere undergraduate synthetic biology competition. The competing teams consist of students who work on a synthetic biology ... Read more
Lnc-ing inflammation to disease

Author: Magagula, L ; Gagliardi, M ; Naidoo, Jerolen ; Mhlanga, Musa Date: Aug 2017 Termed 'master gene regulators' long ncRNAs (lncRNAs) have emerged as the true vanguard of the 'noncoding revolution'. Functioning at a molecular level, in most if not all cellular processes, lncRNAs exert their effects systemically. Thus, ... Read more
lncRNA and gene looping: what’s the connection?

Author: Shibayama, Y ; Fanucchi, Stephanie ; Magagula, L ; Mhlanga, Musa Date: May 2014 Recent functional studies have unveiled the significant role chromatin topology plays in gene regulation. Several lines of evidence suggest genes access necessary factors for transcription by forming chromatin loops. A clearer picture of the ... Read more
PALM and STORM: unlocking live-cell super-resolution

Author: Henriques, R ; Griffiths, C ; Rego, EH ; Mhlanga, Musa Date: May 2011 Live-cell fluorescence light microscopy has emerged as an important tool in the study of cellular biology. The development of fluorescent markers in parallel with super-resolution imaging systems has pushed light microscopy into the realm of ... Read more
PALM and STORM: what hides beyond the Rayleigh limit?

Author: Henriques, R ; Mhlanga, Musa Date: Jun 2009 Super-resolution imaging allows the imaging of fluorescently labeled probes at a resolution of just tens of nanometers, surpassing classic light microscopy by at least one order of magnitude. Recent advances such as the development of ... Read more
QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ

Author: Henriques, R ; Lelek, M ; Fornasiero, EF ; Valtorta, F ; Zimmer, C ; Mhlanga, Musa Date: May 2010 QuickPALM in conjunction with the acquisition of control features provides a complete solution for the acquisition, reconstruction and visualization of 3D PALM or STORM images, achieving resolutions of ~40 nm in real time. This software package ... Read more
Stability and structural properties of gene regulation networks with coregulation rules

Author: Warrell, Jonathan ; Mhlanga, Musa Date: May 2017 Coregulation of the expression of groups of genes has been extensively demonstrated empirically in bacterial and eukaryotic systems. Such coregulation can arise through the use of shared regulatory motifs, which allow the coordinated expression ... Read more
Super-resolution microscopy reveals a preformed NEMO lattice structure that is collapsed in incontinentia pigmenti

Author: Scholefield, Janine ; Henriques, R ; Savulescu, AF ; Fontan, E ; Boucharlat, A ; Laplantine, E ; Smahi, A ; Israel, A ; Agou, F ; Mhlanga, Musa Date: Sep 2016 The NF- B pathway has critical roles in cancer, immunity and inflammatory responses. Understanding the mechanism(s) by which mutations in genes involved in the pathway cause disease has provided valuable insight into its regulation, yet many ... Read more
Synthetic biology's tall order: Reconstruction of 3D, super resolution images of single molecules in real-time

Author: Henriques, R ; Lelek, M ; Fornasiero, EF ; Valtorta, F ; Zimmer, C ; Mhlanga, Musa Date: 31 Aug 2010 Super-resolution microscopy using photoswitchable molecules requires fast and efficient software for single molecule detection, localization, drift correction and visualization. However, there is still a dearth of freely available, flexible ... Read more
Systems biology tools to understand the role of host miRNAs in infection: a closer look at HIV

Author: Naidoo, Jerolen ; Brackin, R ; Khutlang, Rethabile ; Savulescu, A ; Mhlanga, Musa Date: Jun 2014 The discovery of mammalian microRNAs (miRNAs) has greatly enhanced our appreciation for the complexity associated with the regulation of the mammalian transcriptional landscape. Endogenous miRNA pathways mediate the targeted and subtle ... Read more
Transcriptome dysregulation by anthrax lethal toxin plays a key role in induction of human endothelial cell cytotoxicity

Author: Rolando, M ; Stefani, C ; Flatau, G ; Auberger, P ; Mettouchi, A ; Mhlanga, Musa ; Rapp, U ; Galmiche, A ; Lemichez, E Date: Jul 2010 The authors have investigated how Bacillus anthracis lethal toxin (LT) triggers caspase-3 activation and the formation of thick actin cables in human endothelial cells. By DNA array analysis they show that LT has a major impact on the cell ... Read more
Visualization of enhancer-derived noncoding RNA

Author: Shibayama, Y ; Fanucchi, Stephanie ; Mhlanga, Musa Date: 2017 Enhancers are principal regulators that allow spatiotemporal tissue-specifi c control of gene expression. While mounting evidence suggests that enhancer-derived long noncoding RNAs (long ncRNAs), including enhancer RNAs (eRNAs), are an important ... Read more
Wits Post Graduate Symposium Poster - iGEM

Author: Millroy, L ; Madigoe, E ; Kowalenko, R ; Rubin, R ; Rumbold, K ; Sherwell, D ; Sparrow, RW ; Mhlanga, Musa ; Weinberg, M Date: Aug 2010 This document is a poster providing details of the 2010 International Genetically Engineered Machines (iGEM) competition. The origins of the competition, as well as criteria, judging, sponsors, topic and team, are briefly described. Read more

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