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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/4221

Title: Synthetic biology's tall order: Reconstruction of 3D, super resolution images of single molecules in real-time
Authors: Henriques, R
Lelek, M
Fornasiero, EF
Valtorta, F
Zimmer, C
Mhlanga, MM
Keywords: Synthetic biology
Super resolution images
QuickPALM
3D Images
Issue Date: 31-Aug-2010
Publisher: CSIR
Citation: Henriques, R, Lelek, M, Fornasiero, EF. 2010. Synthetic biology's tall order: Reconstruction of 3D, super resolution images of single molecules in real-time. CSIR 3rd Biennial Conference: Science Real and Relevant, CSIR International Convention Centre, Pretoria, South Africa, 30 August – 1 September 2010, pp 7
Abstract: Super-resolution microscopy using photoswitchable molecules requires fast and efficient software for single molecule detection, localization, drift correction and visualization. However, there is still a dearth of freely available, flexible and easy-to-use reconstruction software coupled with image acquisition. Here, we present QuickPALM, an Image plugin, enabling real-time reconstruction of 3D super-resolution images during acquisition and drift correction. We illustrate its application by reconstructing Cy5-labelled microtubules with a resolution of 40-50 nm
Description: CSIR 3rd Biennial Conference: Science Real and Relevant, CSIR International Convention Centre, Pretoria, South Africa, 30 August – 1 September 2010
URI: http://hdl.handle.net/10204/4221
Appears in Collections:CSIR Conference 2010
Open source
General science, engineering & technology
General research interest

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