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Browsing by Author "Mancama, Dalubuhle T"

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Characterization, modelling and docking studies of Plasmodium falciparum kinase PfCDPK4

Author: Makungo, T ; Tsekoa, Tsepo L ; Theron, Anjo ; Mancama, Dalubuhle T ; Van Ree, T Date: Apr 2019 Characterization, modelling and docking studies of Plasmodium falciparum kinase PfCDPK4: The increasing incidence of Plasmodium strains that are resistant to current frontline antimalarial drugs has become one of the greatest challenges of ... Read more
Characterization, modelling and docking studies of Plasmodium falciparum kinase PfCDPK4

Author: Makungo, T ; Tsekoa, Tsepo L ; Theron, Anjo ; Mancama, Dalubuhle T ; Van Ree, T Date: Apr 2019 Characterization, modelling and docking studies of Plasmodium falciparum kinase PfCDPK4: The increasing incidence of Plasmodium strains that are resistant to current frontline antimalarial drugs has become one of the greatest challenges of ... Read more
Development and application of approaches in protein profiling for Nguni cattle

Author: Buthelezi, Sindisiwe G ; Mathabe, P ; Mancama, Dalubuhle T Date: 01 Sep 2010 As a consequence of natural selection in stressful environmental conditions, the Nguni cattle have been reported to be metabolically superior under unfavourable conditions, thus indicating an adaptive measure to survive times of poor feed ... Read more
Development of thermoresponsive poly(propylene-g-N-isopropylacrylamide) non-woven 3D scaffold for smart cell culture using oxyfluorination-assisted graft polymerisation

Author: Chetty, AS ; Vargha, V ; Maity, Arjun ; Moolman, FS ; Rossouw, C ; Anandjiwala, R ; Boguslavsky, L ; Mancama, Dalubuhle T ; Focke, WW Date: Feb 2013 Growing cells on 3D scaffolds is far superior to the conventional 2D monolayer culture method. In this study, a novel 3D thermoresponsive poly(propylene-g-N-isopropylacrylamide) (PP-g-PNIPAAm) nonwoven fabric (gNWF) was developed for cell ... Read more
Elucidating antimalarial drug targets/mode-of-action by application of system biology technologies

Author: Becker, J ; Mtwisha, L ; Crampton, B ; Mancama, Dalubuhle T Date: Nov 2008 Malaria is one of the world's most important tropical diseases, killing more than a million of an estimated 300-500 million infected people per annum. The species responsible for the most fatalities amongst the genus is Plasmodium falsiparum. ... Read more
Establishing malaria parasite transfection technology in South Africa

Author: Van Brummelen, AC ; Becker, JVW ; Mancama, Dalubuhle T ; Hoppe, HC Date: Jan 2010 The most important contributing factor to the current malaria crisis is the rapid spread of parasite resistance to available anti-malarial drugs. Anti-malarial drug resistance is critical and the need for compounds with novel modes of action ... Read more
Establishing malaria parasite transfection technology in South Africa.

Author: Van Brummelen, AC ; Becker, JVW ; Mancama, Dalubuhle T ; Hoppe, H Date: Jan 2010 In order to establish malaria parasite transfection technology in South Africa, firefly luciferase and green fluorescent protein (GFP) reporter constructs were prepared. In attempt to simplify these constructs, a var intron (PFC0005w), ... Read more
Gel and gel-free approaches for the quantitative characterisation of complex protein mixtures

Author: Buthelezi, Sindisiwe G ; Tsekoa, Tsepo L ; Stoychev, Stoyan H ; Mancama, Dalubuhle T ; Blackburn, J Date: Oct 2012 The research aims to establish a reliable set of methods for profiling proteins in a complex mixture in order to allow for the mining of low abundant species. Read more
In vitro anti-plasmodial activity of Dicoma anomala subsp. gerrardii (Asteraceae): identification of its main active constituent, structure-activity relationship studies and gene expression profiling

Author: Becker, JVW ; Van der Merwe, MM ; Van Brummelen, AC ; Pillay, P ; Crampton, BG ; Mmutlane, EM ; Parkinson, C ; Van Heerden, FR ; Crouch, NR ; Smith, PJ ; Mancama, Dalubuhle T ; Maharaj, VJ Date: Oct 2011 Anti-malarial drug resistance threatens to undermine efforts to eliminate this deadly disease. The resulting omnipresent requirement for drugs with novel modes of action prompted a national consortium initiative to discover new anti-plasmodial ... Read more
Inhibition of Plasmodium falciparum spermidine synthase indicates unique perturbation-specific effects in the transcriptome and proteome

Author: Becker, J ; Mtwisha, L ; Crampton, BG ; Stoychev, Stoyan H ; Van Brummelen, A ; Louw, A ; Birkholtz, L ; Mancama, Dalubuhle T Date: Mar 2009 Antiplasmodial drug discovery efforts are hampered by the lack of an extensive set of novel, validated drug targets. A requirement of these targets (or the pathways in which they function) is that they prove essential for parasite survival. ... Read more
Molecular Modelling of Calcium Dependent Protein Kinase 4 (CDPK4) from Plasmodium falciparum

Author: Tsekoa, Tsepo L ; Makungo, T ; Mancama, Dalubuhle T Date: Jul 2012 Malaria continues to be one of the most serious global health challenges. The increasing incidence of drug resistant Plasmodium strains has emphasised the need for urgent action in the development of new therapeutic strategies against this ... Read more
Optimization of an in vitro system to study the exo-erythrocytic stage of the human malaria parasite, Plasmodium falciparum

Author: Rossouw, C ; Chetty, A ; Moolman, S ; Hoppe, H ; Birkholtz, L ; Mancama, Dalubuhle T Date: Feb 2010 Much research remains to be done in order to understand the Plasmodium-liver interaction process. While previous in vitro studies have focused on two-dimensional (2D) cell culture, it is becoming more evident to researchers that the context ... Read more
Plasmodium falciparum spermidine synthase inhibition results in unique perturbation-specific effects observed on transcript, protein and metabolite levels

Author: Becker, JVW ; Mtwisha, L ; Crampton, BG ; Stoychev, Stoyan H ; Van Brummelen, AC ; Reeksting, S ; Louw, AI ; Birkholtz, LM ; Mancama, Dalubuhle T Date: Jan 2010 This study details the malaria parasite's response to PfSpdSyn inhibition on the transcriptomic, proteomic and metabolic levels. The results corroborate and significantly expand previous functional genomics studies relating to polyamine ... Read more
Profiling of botanical extracts for authentication, detection of adulteration and quality control using UPLC-QTOF-MS

Author: Steenkamp, Paul A ; Steenkamp, Lucia H ; Mancama, Dalubuhle T Date: Jan 2018 Herbs and plant extracts are very popular and are being used by all cultures for healthcare and as food supplements. The correct identification of the required plant material is vital for formulation of final formulas for use by mankind. The ... Read more
A review of the experimental approaches used in clinical studies to evaluate the health benefits of plant food supplements associated with infectious diseases

Author: Naidoo, Dashnie ; Mancama, Dalubuhle T ; Meoni, P ; Maharaj, VJ ; Van Rooyen, S Date: 2015 The objective of this review was to evaluate the experimental approaches used in clinical trials to support the benefit claims of plant food supplements (PFS) with reported activity on infectious diseases. A literature search was conducted ... Read more
Smart 3D cell culture using a thermoresponsive PP nonwoven scaffold

Author: Chetty, A ; Rossouw, C ; Ntsangani, L ; Moolman, S ; Van Wyk, K ; Mancama, Dalubuhle T ; Anandjiwala, R ; Boguslavsky, L ; Vargha, V Date: May 2011 In this paper the grafting method will be described and characterisation of the smart PP-g-PNIPAAm scaffolds by fourier-transform infrared, scanning electron microscopy, x-ray photoelectron spectroscopy, differential scanning calorimetry, ... Read more
Targeted ultra-deep sequencing of a South African Bantu-speaking cohort to comprehensively map and characterize common and novel variants in 65 pharmacologically-related genes

Author: Tshabalala, Elizabeth S ; Choudhury, A ; Beeton-Kempen, Natasha ; Martinson, N ; Ramsay, M ; Mancama, Dalubuhle T Date: Sep 2019 BACKGROUND: African populations are characterised by high genetic diversity, which provides opportunities for discovering and elucidating novel variants of clinical importance, especially those affecting therapeutic outcome. Significantly ... Read more
Thermo-Responsive non-woven scaffolds for ‘‘smart’’ 3D cell culture

Author: Rossouw, CL ; Chetty, A ; Moolman, FS ; Birkholtz, L-M ; Hoppe, H ; Mancama, Dalubuhle T Date: Aug 2012 The thermo-responsive polymer poly(Nisopropylacrylamide) has received widespread attention for its in vitro application in the non-invasive, nondestructive release of adherent cells on two dimensional surfaces. In this study, 3D non-woven ... Read more
UCT943, a next-generation plasmodium falciparum PI4K inhibitor preclinical candidate for the treatment of Malaria

Author: Brunschwig, C ; Lawrence, N ; Taylor, D ; Abay, E ; Njoroge, M ; Basarab, GS ; Le Manach, C ; Paquet, T ; Cabrera, DG ; Mancama, Dalubuhle T Date: Sep 2018 The 2-aminopyridine MMV048 was the first drug candidate inhibiting Plasmodium phosphatidylinositol 4-kinase (PI4K), a novel drug target for malaria, to enter clinical development. In an effort to identify the next generation of PI4K inhibitors, ... Read more

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