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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/3971

Title: Establishing malaria parasite transfection technology in South Africa.
Authors: Van Brummelen, AC
Becker, JVW
Mancama, D
Hoppe, H
Keywords: Plasmodium falciparum
Anti-malarial drug
Anti-malarial drug resistance
Transfection
Malaria
Parasite transfection technology
Biochemistry
Molecular biology
Issue Date: Jan-2010
Citation: Van Brummelen AC, Becker JVW et al. 2010. Establishing malaria parasite transfection technology in South Africa. 22nd South African Society for Biochemistry and Molecular Biology Congress. Bloemfontein, South Africa, 18-20 January 2010, pp 2
Abstract: In order to establish malaria parasite transfection technology in South Africa, firefly luciferase and green fluorescent protein (GFP) reporter constructs were prepared. In attempt to simplify these constructs, a var intron (PFC0005w), previously reported to have bidirectional promoter activity, was utilized to drive expression through two genes (i.e. the antibiotic-resistance gene, human dhfr and the reporter gene) in a head-to-head orientation. In addition, protocols were adjusted by including DNA packaging reagents to improve uptake into the parasite and by using shaking instead of stationary parasite cultures to improve the parasite proliferation and selection rate. Successfully transfected parasites were selected by the antifolate WR99210.
Description: 22nd South African Society for Biochemistry and Molecular Biology Congress. Bloemfontein, South Africa, 18-20 January 2010
URI: http://hdl.handle.net/10204/3971
Appears in Collections:Systems biology
General science, engineering & technology

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