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A discussion of molecular biology methods for protein engineering

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dc.contributor.author Zawaira, A
dc.contributor.author Pooran, A
dc.contributor.author Barichievy, S
dc.contributor.author Chopera, D
dc.date.accessioned 2012-03-28T09:48:09Z
dc.date.available 2012-03-28T09:48:09Z
dc.date.issued 2011-09
dc.identifier.citation Zawaira, A, Pooran, A, Barichievy, S and Chopera, D. 2011. A discussion of molecular biology methods for protein engineering. Molecular Biotechnology, 36pp en_US
dc.identifier.issn 1073-6085
dc.identifier.issn 1559-0305
dc.identifier.uri http://www.springerlink.com/content/675581q2558p0535/
dc.identifier.uri http://hdl.handle.net/10204/5690
dc.description Copyright: 2011 Humana Press. This is an ABSTRACT ONLY. en_US
dc.description.abstract A number of molecular biology techniques are available to generate variants from a particular start gene for eventual protein expression. The authors discuss the basic principles of these methods in a repertoire that may be used to achieve the elemental steps in protein engineering. These include site-directed, deletion and insertion mutagenesis. They provide detailed case studies, drawn from their own experiences, packaged together with conceptual discussions and include an analysis of the techniques presented with regards to their uses in protein engineering. en_US
dc.language.iso en en_US
dc.publisher Humana Press en_US
dc.relation.ispartofseries Workflow;8502
dc.subject Molecular biology en_US
dc.subject Molecular biology methods en_US
dc.subject Protein engineering en_US
dc.subject Protein expression en_US
dc.subject Protein expression methods en_US
dc.subject Mutagenesis en_US
dc.subject Site-directed mutagenesis en_US
dc.subject Insertion mutagenesis en_US
dc.subject Deletion mutagenesis en_US
dc.subject Polymerase chain reaction en_US
dc.subject Molecular cloning en_US
dc.subject Recombinant protein expression en_US
dc.subject Recombinant plasmid en_US
dc.title A discussion of molecular biology methods for protein engineering en_US
dc.type Article en_US
dc.identifier.apacitation Zawaira, A., Pooran, A., Barichievy, S., & Chopera, D. (2011). A discussion of molecular biology methods for protein engineering. http://hdl.handle.net/10204/5690 en_ZA
dc.identifier.chicagocitation Zawaira, A, A Pooran, S Barichievy, and D Chopera "A discussion of molecular biology methods for protein engineering." (2011) http://hdl.handle.net/10204/5690 en_ZA
dc.identifier.vancouvercitation Zawaira A, Pooran A, Barichievy S, Chopera D. A discussion of molecular biology methods for protein engineering. 2011; http://hdl.handle.net/10204/5690. en_ZA
dc.identifier.ris TY - Article AU - Zawaira, A AU - Pooran, A AU - Barichievy, S AU - Chopera, D AB - A number of molecular biology techniques are available to generate variants from a particular start gene for eventual protein expression. The authors discuss the basic principles of these methods in a repertoire that may be used to achieve the elemental steps in protein engineering. These include site-directed, deletion and insertion mutagenesis. They provide detailed case studies, drawn from their own experiences, packaged together with conceptual discussions and include an analysis of the techniques presented with regards to their uses in protein engineering. DA - 2011-09 DB - ResearchSpace DP - CSIR KW - Molecular biology KW - Molecular biology methods KW - Protein engineering KW - Protein expression KW - Protein expression methods KW - Mutagenesis KW - Site-directed mutagenesis KW - Insertion mutagenesis KW - Deletion mutagenesis KW - Polymerase chain reaction KW - Molecular cloning KW - Recombinant protein expression KW - Recombinant plasmid LK - https://researchspace.csir.co.za PY - 2011 SM - 1073-6085 SM - 1559-0305 T1 - A discussion of molecular biology methods for protein engineering TI - A discussion of molecular biology methods for protein engineering UR - http://hdl.handle.net/10204/5690 ER - en_ZA


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