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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/5690

Title: A discussion of molecular biology methods for protein engineering
Authors: Zawaira, A
Pooran, A
Barichievy, S
Chopera, D
Keywords: Molecular biology
Molecular biology methods
Protein engineering
Protein expression
Protein expression methods
Site-directed mutagenesis
Insertion mutagenesis
Deletion mutagenesis
Polymerase chain reaction
Molecular cloning
Recombinant protein expression
Recombinant plasmid
Issue Date: Sep-2011
Publisher: Humana Press
Citation: Zawaira, A, Pooran, A, Barichievy, S and Chopera, D. 2011. A discussion of molecular biology methods for protein engineering. Molecular Biotechnology, 36pp
Series/Report no.: Workflow;8502
Abstract: A number of molecular biology techniques are available to generate variants from a particular start gene for eventual protein expression. The authors discuss the basic principles of these methods in a repertoire that may be used to achieve the elemental steps in protein engineering. These include site-directed, deletion and insertion mutagenesis. They provide detailed case studies, drawn from their own experiences, packaged together with conceptual discussions and include an analysis of the techniques presented with regards to their uses in protein engineering.
Description: Copyright: 2011 Humana Press. This is an ABSTRACT ONLY.
URI: http://www.springerlink.com/content/675581q2558p0535/
ISSN: 1073-6085
Appears in Collections:Human factors
General science, engineering & technology

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