A discussion of molecular biology methods for protein engineering

Abstract

A number of molecular biology techniques are available to generate variants from a particular start gene for eventual protein expression. The authors discuss the basic principles of these methods in a repertoire that may be used to achieve the elemental steps in protein engineering. These include site-directed, deletion and insertion mutagenesis. They provide detailed case studies, drawn from their own experiences, packaged together with conceptual discussions and include an analysis of the techniques presented with regards to their uses in protein engineering.