In order to investigate the possibility of using the FliC display system for biotransformation in B. halodurans, construct pSECNLipC habouring the LipA gene from Geobacillus thermoleovorans was designed and evaluated in shake flasks and batch fermentations. Lipases have been well characterised and play a role in a number of processes which include detergents, glycerolysis of fats and oils, direct esterification, chiral resolution and acylate synthesis (Litthauer et al. 2002). The use of lipases immobilized on the cell surface of Staphylococcus carnosus for biotransformation has been demonstrated using the fibronectin binding protein B fused to the Staphylococcus hyicus lipase
Reference:
Ramchuran, SO, Berger, E, Du Plessis, E, Crampton, BG and Louw, ME. 2006. Recombinant lipase immobilised on the cell surface of Bacillus halodurans Alk 36 exploiting the FliC protein. 4TH Recombinant protein production conference, Barcelona, Spain, 21–23 September 2006, pp 2
Ramchuran, S. O., Berger, E., Du Plessis, E., Crampton, B., & Louw, M. (2006). Recombinant lipase immobilised on the cell surface of Bacillus halodurans Alk 36 exploiting the FliC protein. BioMed Central. http://hdl.handle.net/10204/3350
Ramchuran, Santosh O, E Berger, E Du Plessis, BG Crampton, and ME Louw. "Recombinant lipase immobilised on the cell surface of Bacillus halodurans Alk 36 exploiting the FliC protein." (2006): http://hdl.handle.net/10204/3350
Ramchuran SO, Berger E, Du Plessis E, Crampton B, Louw M, Recombinant lipase immobilised on the cell surface of Bacillus halodurans Alk 36 exploiting the FliC protein; BioMed Central; 2006. http://hdl.handle.net/10204/3350 .
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