ResearchSpace
→
Browsing by Subject

Browsing by Subject "Biocatalysis"

Now showing items 1-20 of 22

Next Page

Advances in enzyme immobilisation

Author: Brady, D ; Jordaan, J Date: 10 Jul 2009 Improvements in current carrier-based immobilisation strategies have been developed using hetero-functionalised supports that enhance the binding efficacy and stability through multipoint attachment. New commercial resins (Sepabeads) exhibit ... Read more
Application of biocatalysis in synthetic chemistry

Author: Brady, D ; Molefe, M ; Steenkamp, Lucia H ; Parkinson, C ; Jordaan, J ; Visser, Daniel F ; Simpson, C ; Chhiba, Varsha P ; Mathiba, K Date: Nov 2006 It was found that the nitrile hydratases in microorganisms isolated from around the world had infra-species amino acid sequence differences, and this provided an explanation for the variability of nitrile substrate usage within the species. ... Read more
Bacterial nitrilases and their regulation

Author: Chhiba, Varsha P ; Van der Westhuyzen, Christiaan W ; Bode, M ; Brady, D Date: Jun 2019 Commercially, nitrilases are valuable biocatalysts capable of converting a diverse range of nitriles to carboxylic acids for the greener synthesis of chemicals and pharmaceuticals. Nitrilases are widespread in nature and are both important ... Read more
Biocatalytic preparation of 5-methyluridine (5-MU)

Author: Gordon, GER ; Visser, Daniel F ; Bode, ML ; Brady, D Date: Nov 2008 The potential of chemo-enzymatic methods to produce active pharmaceutical ingredients (APIs) such as stavudine (d4T) and zidovudine (AZT) has been demostrated during this investigation. The effect of increasing reactor productivity to ... Read more
Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36

Author: Visser, Daniel F ; Hennessy, F ; Rashamuse, KJ ; Louw, ME ; Brady, D Date: Mar 2010 A purine nucleoside phosphorylase from the alkaliphile Bacillus halodurans Alk36 has been cloned and over expressed in E. coli. The purified enzyme had a kcat of 2.03 x 10-9 s-1 and a km of 206 µM on guanosine. The optimal pH range was between ... Read more
Decision making in the development of a biocatalytic route for resolution of S-naproxen: from screening to scale-up

Author: Steenkamp, Lucia H ; Brady, D Date: Jun 2010 The non-steroidal anti-inflammatory drug naproxen is most effective as the single S-naproxen enantiomer. However, typical synthetic routes to naproxen yield the racemate of both the R and the S stereoisomers. Biocatalysts can be used to resolve ... Read more
Development of a two-step “green” synthesis for (-)-ambafuran production

Author: Steenkamp, Lucia H Date: 01 Sep 2010 The main objective of the development of a two-step “green” synthesis for (-)-ambafuran production is to find an alternative synthesis of (-) Ambrox from sclareol, to use a bioconversion or biocatalysis route, and that it results in a natural ... Read more
Diamination by n-coupling using a commercial laccase

Author: Wellington, Kevin W ; Steenkamp, P ; Brady, D Date: Feb 2010 Nuclear diamination of p-hydrobenzoquinones with aromatic and aliphatic primary amines was catalyzed by a immobilised commercial laccase, Denilite II Base, from Novozymes. The amine and the p-hydrobenzoquinone was reacted under mild conditions ... Read more
Dimethylformamide is a novel nitrilase inducer in Rhodococcus rhodochrous

Author: Chhiba, Varsha P ; Mathiba, Kgama ; Van der Westhuyzen, Christiaan W ; Steenkamp, Paul A ; Rashamuse, JK ; Stoychev, Stoyan H ; Bode, ML ; Brady, D Date: Dec 2018 Nitrilases are of commercial interest in the selective synthesis of carboxylic acids from nitriles. Nitrilase induction was achieved here in three bacterial strains through the incorporation of a previously unrecognised and inexpensive nitrilase ... Read more
Enantioselective biocatalytic hydrolysis of ß-aminonitriles to ß-amino-amides using Rhodococcus rhodochrous ATCC BAA-870

Author: Chhiba, Varsha P ; Bode, M ; Mathiba, K ; Kwezi, Lusisizwe ; Brady, D Date: Apr 2012 A range of ß-aminonitriles (3-amino-3-phenylpropanenitrile and derivatives) were synthesised by reaction of various benzonitriles with acetonitrile and subsequent reduction of the resulting acrylonitrile products. These compounds were hydrolysed ... Read more
Gas chromatographic profiling of the biocatalytic conversion of sclareol to ambradiol by Hyphozyma roseoniger

Author: Ncube, EN ; Mathiba, Kgama ; Steenkamp, Lucia H ; Dubery, IA Date: Oct 2021 Hyphozyma roseoniger is a filamentous yeast used for its biocatalytic ability to convert sclareol, a plant diterpenoid to ambradiol, an intermediate to ambrafuran, which is a sought-after fixative in the perfume industry. Metabolite profiling ... Read more
Green chemistry: highly selective biocatalytic hydrolysis of nitrile compounds

Author: Brady, D ; Dube, N ; Petersen, R Date: 28 Feb 2006 Nitrile substrate profiles were determined for a wide range of microorganisms. Isolated bacteria and yeast were enriched on nitriles as the sole nitrogen source. Aliphatic and aromatic nitriles were investigated, as well as structurally related ... Read more
High yielding cascade enzymatic synthesis of 5-methyluridine using a novel Purine Nucleoside Phosphorylase, from Bacillus halodurans Alk36

Author: Vissser, D ; Hennessy, F ; Rashamuse, K ; Van Zyl, P ; Gordon, G ; Mathiba, K ; Bode, M ; Pletschke, B ; Brady, D Date: Sep 2010 5-Methyluridine(5-MU) is a non-natural nucleoside that can be used as an intermediate in the synthesis of thymidine, and in the synthesis of nucleoside analogues AZT and stavudine, both of which are used in Highly Active Anti-Retroviral ... Read more
Immobilisation and characterisation of biocatalytic co-factor recycling enzymes, glucose dehydrogenase and NADH oxidase, on aldehyde functional ReSynTM polymer microspheres

Author: Twala, BV ; Sewell, BT ; Jordaan, J Date: Mar 2012 The use of enzymes in industrial applications is limited by their instability, cost and difficulty in their recovery and re-use. Immobilisation is a technique which has been shown to alleviate these limitations in biocatalysis. Here we describe ... Read more
An integrated chemo-enzymatic route for preparation of ß-thymidine, a key intermediate in the preparation of antiretrovirals

Author: Gordon, GER ; Bode, ML ; Visser, Daniel F ; Lepuru, MJ ; Zeevaart, JG ; Ragubeer, N ; Ratsaka, M ; Walwyn, DR ; Brady, D Date: Jan 2011 A chemo-enzymatic method for production of ß-thymidine, an intermediate in the synthesis of antiretrovirals, is described. Guanosine and thymine were converted by means of enzymatic transglycosylation to yield 5-methyluridine (5-MU), which ... Read more
A Laccase-catalysed one-pot synthesis of aminonaphthoquinones and their anticancer activity

Author: Wellington, Kevin W ; Kolesnikova, NI Date: May 2012 Nuclear monoamination of a 1,4-naphthohydroquinone with primary aromatic amines was catalysed by the commercial laccase, Novozym 51003, from Novozymes to afford aminonaphthoquinones. The synthesis was accomplished by reacting a mixture of the ... Read more
Naproxen: from skunk work to scale-up

Author: Steenkamp, Lucia H ; Brady, D Date: Nov 2008 The presentation starts with a comparison between Naproxen and Aspirin. The characteristics and properties of Naproxen are explained, as well as the biocatalysis and the screening of the enzyme activity and selectivity. It ends with a discussion ... Read more
A one-pot laccase-catalysed synthesis of 5,6-dihydroxylated benzo[b]furans and catechol derivatives, and their anticancer activity

Author: Wellington, Kevin W ; Qwebani-Ogunleye, T ; Kolesnikova, NI ; Brady, D ; De Koning, CB Date: Apr 2013 A commercial laccase, Suberase® from Novozymes, was used to catalyse the synthesis of 5,6-dihydroxylated benzo[b]furans and catechol derivatives. The yields were, in some cases, similar to or better than that obtained by other enzymatic, ... Read more
A one-pot laccase-catalysed synthesis of coumestan derivatives and their anticancer activity

Author: Qwebani-Ogunleye, Tozama ; Kolesnikova, Natalia I ; Steenkamp, Paul A ; De Koning, CB ; Brady, D ; Wellington, Kevin W Date: Dec 2016 Suberase®, a commercial laccase from Novozymes, was used to catalyse the synthesis of coumestans. The yields, in some cases, were similar to or better than that obtained by other enzymatic, chemical or electrochemical syntheses. The compounds ... Read more

Now showing items 1-20 of 22

Next Page

Browse

All of ResearchSpace

Quick Links

Legislation and compliance

General Enquiries

Tel: + 27 12 841 2911
Email: callcentre@csir.co.za

Physical Address
Meiring Naudé Road
Brummeria
Pretoria
South Africa

Postal Address
PO Box 395
Pretoria 0001
South Africa

Social Connect

Copyright © CSIR 2017. All Rights Reserved

Resources on this site are free to download and reuse according to associated licensing provision. Please read the terms and conditions of usage of each resource.