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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/4008

Title: Advances in enzyme immobilisation
Authors: Brady, D
Jordaan, J
Keywords: Enzyme
Biocatalysis
Biocatalyst
Immobilisation enzyme
Sepabeads
Immobilisation
Hetero-functionalised supports
Encapsulation
Entrapment
Support based immobilisation
Self immobilisation
Issue Date: 10-Jul-2009
Publisher: Springer Netherlands
Citation: Brady, D and Jordaan, J 2009. Advances in enzyme immobilisation. Biotechnology Letters, Vol. 31(11), pp 1639-1650
Abstract: Improvements in current carrier-based immobilisation strategies have been developed using hetero-functionalised supports that enhance the binding efficacy and stability through multipoint attachment. New commercial resins (Sepabeads) exhibit improved protein binding capacity. Novel methods of enzyme self immobilisation have been developed (CLEC, CLEA, Spherezyme), as well as carrier materials (Dendrispheres), encapsulation (PEI Microspheres), and entrapment. Apart from retention, recovery and stabilisation, other advantages to enzyme immobilisation have emerged, such as enhanced enzyme activity, modification of substrate selectivity and enantioselectivity, and multi-enzyme reactions. These advances promise to enhance the roles of immobilisation enzymes in industry, while opening the door for novel applications.
Description: Copyright: 2009 Springer Netherlands. This is the author’s post print version. It is posted here by permission of Springer Netherlands for your personal use. Not for redistribution. The definitive version has been published in the Journal of Biotechnology Letters, Vol. 31(11), pp 1639-1650
URI: http://www.springerlink.com/content/g71665208l264352/fulltext.html
http://www.springerlink.com/content/g71665208l264352/
http://hdl.handle.net/10204/4008
ISSN: 0141-5492
Appears in Collections:Enzyme technologies
General science, engineering & technology

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