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Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures

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dc.contributor.author Wesley-Smith, J
dc.contributor.author Berjak, P
dc.contributor.author Pammenter, NW
dc.contributor.author Walters, Chavon R
dc.date.accessioned 2014-09-05T12:53:31Z
dc.date.available 2014-09-05T12:53:31Z
dc.date.issued 2014-03
dc.identifier.citation Wesley-Smith, J, Berjak, P, Pammenter, N.W and Walters, C.R. 2014. Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures. Annals of Botany, vol. 113(4), pp 695-709 en_US
dc.identifier.issn 0305-7364
dc.identifier.uri http://aob.oxfordjournals.org/content/early/2013/12/23/aob.mct284.abstract
dc.identifier.uri http://hdl.handle.net/10204/7649
dc.description Copyright: 2014 Oxford University Press. This is an ABSTRACT ONLY. The definitive version is published in Annals Botany, vol. 113(4), pp 695-709 en_US
dc.description.abstract Cryopreservation is the only long-term conservation strategy available for germplasm of recalcitrant-seeded species. Efforts to cryopreserve this form of germplasm are hampered by potentially lethal intracellular freezing events; thus, it is important to understand the relationships among cryo-exposure techniques, water content, structure and survival. en_US
dc.language.iso en en_US
dc.publisher Oxford University Press (OUP) en_US
dc.relation.ispartofseries Workflow;13400
dc.subject Acer saccharinum en_US
dc.subject Cooling rate en_US
dc.subject Cryopreservation en_US
dc.subject Electron microscopy en_US
dc.subject Embryonic axes en_US
dc.subject Freeze-fracture en_US
dc.subject Freez-substitution en_US
dc.subject Ice crystal distribution en_US
dc.subject Ice crystal size en_US
dc.subject Recalcitrant seeds en_US
dc.subject Warming rate en_US
dc.subject Water content en_US
dc.title Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures en_US
dc.type Article en_US
dc.identifier.apacitation Wesley-Smith, J., Berjak, P., Pammenter, N., & Walters, C. R. (2014). Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures. http://hdl.handle.net/10204/7649 en_ZA
dc.identifier.chicagocitation Wesley-Smith, J, P Berjak, NW Pammenter, and Chavon R Walters "Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures." (2014) http://hdl.handle.net/10204/7649 en_ZA
dc.identifier.vancouvercitation Wesley-Smith J, Berjak P, Pammenter N, Walters CR. Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures. 2014; http://hdl.handle.net/10204/7649. en_ZA
dc.identifier.ris TY - Article AU - Wesley-Smith, J AU - Berjak, P AU - Pammenter, NW AU - Walters, Chavon R AB - Cryopreservation is the only long-term conservation strategy available for germplasm of recalcitrant-seeded species. Efforts to cryopreserve this form of germplasm are hampered by potentially lethal intracellular freezing events; thus, it is important to understand the relationships among cryo-exposure techniques, water content, structure and survival. DA - 2014-03 DB - ResearchSpace DP - CSIR KW - Acer saccharinum KW - Cooling rate KW - Cryopreservation KW - Electron microscopy KW - Embryonic axes KW - Freeze-fracture KW - Freez-substitution KW - Ice crystal distribution KW - Ice crystal size KW - Recalcitrant seeds KW - Warming rate KW - Water content LK - https://researchspace.csir.co.za PY - 2014 SM - 0305-7364 T1 - Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures TI - Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures UR - http://hdl.handle.net/10204/7649 ER - en_ZA


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