Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures
Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures
Cryopreservation is the only long-term conservation strategy available for germplasm of recalcitrant-seeded species. Efforts to cryopreserve this form of germplasm are hampered by potentially lethal intracellular freezing events; thus, it is important to understand the relationships among cryo-exposure techniques, water content, structure and survival.
Reference:
Wesley-Smith, J, Berjak, P, Pammenter, N.W and Walters, C.R. 2014. Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures. Annals of Botany, vol. 113(4), pp 695-709
Wesley-Smith, J., Berjak, P., Pammenter, N., & Walters, C. R. (2014). Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures. http://hdl.handle.net/10204/7649
Wesley-Smith, J, P Berjak, NW Pammenter, and Chavon R Walters "Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures." (2014) http://hdl.handle.net/10204/7649
Wesley-Smith J, Berjak P, Pammenter N, Walters CR. Intracellular ice and cell survival in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum: an ultrastructural study of factors affecting cell and ice structures. 2014; http://hdl.handle.net/10204/7649.
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