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dc.contributor.author Kfir, R
dc.contributor.author Hilner, C
dc.contributor.author Du Preez, M
dc.contributor.author Bateman, B
dc.date.accessioned 2007-06-12T08:11:34Z
dc.date.available 2007-06-12T08:11:34Z
dc.date.issued 1995
dc.identifier.citation Kfir, R, et al. 1995. Studies evaluating the applicability of utilizing the same concentration techniques for the detection of protozoan parasites and viruses in water. Water Science and Technology, vol. 31, 06 May, pp 417-423 en
dc.identifier.issn 0273-1223
dc.identifier.uri http://hdl.handle.net/10204/596
dc.description Copyright: 1995 Pergamon-Elsevier Science Ltd en
dc.description.abstract In this study concentration techniques regularly used for viral detection; i.e. flat-bed ultra-filtration and filterite cartridge filtration, were evaluated for their efficacies in the recovery of protozoan parasites from water. Recovery of cysts was studied using tap water seeded with Giardia muris cysts and compared to methods designed for the detection of protozoan parasites. Recovery of cysts utilizing 1.2 mu m membrane filters was 11.1% (4.5-23%) compared to 11.6% (2.7-25.5%) with ultra-filtration (pore size 46-50 Angstrom, with a molecular cut off of 50 000 Daltons). Comparison of these methods for the isolation of Giardia cysts and Cryptosporidium oocysts from environmental water samples also indicated a similar efficacy. The recovery of cysts from 1001 of seeded samples using a Cuno wynd cartridge filter was 12.2% (1.6-45%) compared to 13.4% (5-24.2%) using a Filterite cartridge filter. Although the results indicated similar recovery efficacies for these two methods, use of Filterite resulted in a more consistent recovery rate. This study also indicated that the use of cartridge filters for the processing of large volume water samples (1001) showed a slightly better recovery efficacy than the flat-bed filtration technique which limits sample volume to about 101. This study shows that concentration techniques utilised for the isolation of enteric viruses can also be applied for the detection of protozoan parasites from water. This procedure allows for co-analysis of both viruses and protozoan parasites and provides a more rapid and cost-effective evaluation of water quality. en
dc.language.iso en en
dc.publisher Pergamon-Elsevier Science Ltd en
dc.subject Giardia en
dc.subject Cryptosporidium en
dc.subject Enteric viruses en
dc.subject Flat bed filtration en
dc.subject Cartridge filter en
dc.subject Ultra-filtration en
dc.title Studies evaluating the applicability of utilizing the same concentration techniques for the detection of protozoan parasites and viruses in water en
dc.type Article en


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