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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/596

Title: Studies evaluating the applicability of utilizing the same concentration techniques for the detection of protozoan parasites and viruses in water
Authors: Kfir, R
Hilner, C
Du Preez, M
Bateman, B
Keywords: Giardia
Cryptosporidium
Enteric viruses
Flat bed filtration
Cartridge filter
Ultra-filtration
Issue Date: 1995
Publisher: Pergamon-Elsevier Science Ltd
Citation: Kfir, R, et al. 1995. Studies evaluating the applicability of utilizing the same concentration techniques for the detection of protozoan parasites and viruses in water. Water Science and Technology, vol. 31, 06 May, pp 417-423
Abstract: In this study concentration techniques regularly used for viral detection; i.e. flat-bed ultra-filtration and filterite cartridge filtration, were evaluated for their efficacies in the recovery of protozoan parasites from water. Recovery of cysts was studied using tap water seeded with Giardia muris cysts and compared to methods designed for the detection of protozoan parasites. Recovery of cysts utilizing 1.2 mu m membrane filters was 11.1% (4.5-23%) compared to 11.6% (2.7-25.5%) with ultra-filtration (pore size 46-50 Angstrom, with a molecular cut off of 50 000 Daltons). Comparison of these methods for the isolation of Giardia cysts and Cryptosporidium oocysts from environmental water samples also indicated a similar efficacy. The recovery of cysts from 1001 of seeded samples using a Cuno wynd cartridge filter was 12.2% (1.6-45%) compared to 13.4% (5-24.2%) using a Filterite cartridge filter. Although the results indicated similar recovery efficacies for these two methods, use of Filterite resulted in a more consistent recovery rate. This study also indicated that the use of cartridge filters for the processing of large volume water samples (1001) showed a slightly better recovery efficacy than the flat-bed filtration technique which limits sample volume to about 101. This study shows that concentration techniques utilised for the isolation of enteric viruses can also be applied for the detection of protozoan parasites from water. This procedure allows for co-analysis of both viruses and protozoan parasites and provides a more rapid and cost-effective evaluation of water quality.
Description: Copyright: 1995 Pergamon-Elsevier Science Ltd
URI: http://hdl.handle.net/10204/596
ISSN: 0273-1223
Appears in Collections:Pollution and waste
Water resources and human health
General science, engineering & technology

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