dc.contributor.author |
Genthe, Bettina
|
en_US |
dc.contributor.author |
Gericke, M
|
en_US |
dc.contributor.author |
Bateman, B
|
en_US |
dc.contributor.author |
Mjoli, N
|
en_US |
dc.contributor.author |
Kfir, R
|
en_US |
dc.date.accessioned |
2007-03-26T10:24:02Z |
en_US |
dc.date.accessioned |
2007-06-07T10:10:01Z |
|
dc.date.available |
2007-03-26T10:24:02Z |
en_US |
dc.date.available |
2007-06-07T10:10:01Z |
|
dc.date.copyright |
|
en_US |
dc.date.issued |
1995 |
en_US |
dc.identifier.citation |
Genthe, B, et al. 1995. Detection of enteric Adenoviruses in South-African waters using gene probes. Water Science and Technology, vol. 31, 06 May, pp 345-350 |
en_US |
dc.identifier.issn |
0273-1223 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/10204/2047
|
en_US |
dc.identifier.uri |
http://hdl.handle.net/10204/2047
|
|
dc.description.abstract |
Gene probes developed locally for both enteric Adenoviruses 40 and 41 were used to determine whether these viruses were present in both raw and treated waters. Approximately sixty water samples were concentrated by ultra filtration and analysed directly for the presence of enteric adenoviruses. Three pre-treatment techniques, namely sephadex columns, cellulose fibre and Geneclean(TM) were tested for the removal of inhibitory substances from concentrated water samples. The effect of chlorine treatment on viral detection using gene probe hybridization was also examined by exposing adenoviruses to chlorine concentrations of up to 20mg/l for 1 hour. Enteric adenoviruses were detected in up to 59% of both raw and treated waters analysed, Cellulose fibre and Geneclean(TM) were found to successfully remove inhibitory substances from concentrated raw waters. Viral I NA was detected after exposure to a range of chlorine concentrations indicating that the viruses detected in the treated waters may have been inactivated virus particles. |
en_US |
dc.format.extent |
296994 bytes |
en_US |
dc.format.mimetype |
application/pdf |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Pergamon-Elsevier Science Ltd |
en_US |
dc.rights |
Copyright: 1995 Pergamon-Elsevier Science Ltd |
en_US |
dc.source |
|
en_US |
dc.subject |
Gene probes |
en_US |
dc.subject |
Enteric adenoviruses |
en_US |
dc.subject |
Sephadex columns |
en_US |
dc.subject |
Cellulose fibre |
en_US |
dc.subject |
Geneclean(TM) |
en_US |
dc.subject |
Removal of inhibitory substances |
en_US |
dc.subject |
Environmental sciences |
en_US |
dc.subject |
Water resources |
en_US |
dc.title |
Detection of enteric Adenoviruses in South-African waters using gene probes |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
Genthe, B., Gericke, M., Bateman, B., Mjoli, N., & Kfir, R. (1995). Detection of enteric Adenoviruses in South-African waters using gene probes. http://hdl.handle.net/10204/2047 |
en_ZA |
dc.identifier.chicagocitation |
Genthe, Bettina, M Gericke, B Bateman, N Mjoli, and R Kfir "Detection of enteric Adenoviruses in South-African waters using gene probes." (1995) http://hdl.handle.net/10204/2047 |
en_ZA |
dc.identifier.vancouvercitation |
Genthe B, Gericke M, Bateman B, Mjoli N, Kfir R. Detection of enteric Adenoviruses in South-African waters using gene probes. 1995; http://hdl.handle.net/10204/2047. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Genthe, Bettina
AU - Gericke, M
AU - Bateman, B
AU - Mjoli, N
AU - Kfir, R
AB - Gene probes developed locally for both enteric Adenoviruses 40 and 41 were used to determine whether these viruses were present in both raw and treated waters. Approximately sixty water samples were concentrated by ultra filtration and analysed directly for the presence of enteric adenoviruses. Three pre-treatment techniques, namely sephadex columns, cellulose fibre and Geneclean(TM) were tested for the removal of inhibitory substances from concentrated water samples. The effect of chlorine treatment on viral detection using gene probe hybridization was also examined by exposing adenoviruses to chlorine concentrations of up to 20mg/l for 1 hour. Enteric adenoviruses were detected in up to 59% of both raw and treated waters analysed, Cellulose fibre and Geneclean(TM) were found to successfully remove inhibitory substances from concentrated raw waters. Viral I NA was detected after exposure to a range of chlorine concentrations indicating that the viruses detected in the treated waters may have been inactivated virus particles.
DA - 1995
DB - ResearchSpace
DP - CSIR
KW - Gene probes
KW - Enteric adenoviruses
KW - Sephadex columns
KW - Cellulose fibre
KW - Geneclean(TM)
KW - Removal of inhibitory substances
KW - Environmental sciences
KW - Water resources
LK - https://researchspace.csir.co.za
PY - 1995
SM - 0273-1223
T1 - Detection of enteric Adenoviruses in South-African waters using gene probes
TI - Detection of enteric Adenoviruses in South-African waters using gene probes
UR - http://hdl.handle.net/10204/2047
ER -
|
en_ZA |