Detection of enteric Adenoviruses in South-African waters using gene probes

Abstract

Gene probes developed locally for both enteric Adenoviruses 40 and 41 were used to determine whether these viruses were present in both raw and treated waters. Approximately sixty water samples were concentrated by ultra filtration and analysed directly for the presence of enteric adenoviruses. Three pre-treatment techniques, namely sephadex columns, cellulose fibre and Geneclean(TM) were tested for the removal of inhibitory substances from concentrated water samples. The effect of chlorine treatment on viral detection using gene probe hybridization was also examined by exposing adenoviruses to chlorine concentrations of up to 20mg/l for 1 hour. Enteric adenoviruses were detected in up to 59% of both raw and treated waters analysed, Cellulose fibre and Geneclean(TM) were found to successfully remove inhibitory substances from concentrated raw waters. Viral I NA was detected after exposure to a range of chlorine concentrations indicating that the viruses detected in the treated waters may have been inactivated virus particles.