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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/2047

Title: Detection of enteric Adenoviruses in South-African waters using gene probes
Authors: Genthe, B
Gericke, M
Bateman, B
Mjoli, N
Kfir, R
Keywords: Gene probes
Enteric adenoviruses
Sephadex columns
Cellulose fibre
Removal of inhibitory substances
Environmental sciences
Water resources
Issue Date: 1995
Publisher: Pergamon-Elsevier Science Ltd
Citation: Genthe, B, et al. 1995. Detection of enteric Adenoviruses in South-African waters using gene probes. Water Science and Technology, vol. 31, 06 May, pp 345-350
Abstract: Gene probes developed locally for both enteric Adenoviruses 40 and 41 were used to determine whether these viruses were present in both raw and treated waters. Approximately sixty water samples were concentrated by ultra filtration and analysed directly for the presence of enteric adenoviruses. Three pre-treatment techniques, namely sephadex columns, cellulose fibre and Geneclean(TM) were tested for the removal of inhibitory substances from concentrated water samples. The effect of chlorine treatment on viral detection using gene probe hybridization was also examined by exposing adenoviruses to chlorine concentrations of up to 20mg/l for 1 hour. Enteric adenoviruses were detected in up to 59% of both raw and treated waters analysed, Cellulose fibre and Geneclean(TM) were found to successfully remove inhibitory substances from concentrated raw waters. Viral I NA was detected after exposure to a range of chlorine concentrations indicating that the viruses detected in the treated waters may have been inactivated virus particles.
URI: http://hdl.handle.net/10204/2047
ISSN: 0273-1223
Appears in Collections:Pollution and waste
Water resources and human health
Coastal and marine systems
General science, engineering & technology

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