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Show simple item record Berger, DK en_US Oelofse, D en_US Arendse, MS en_US Du Plessis, E en_US Dubery, IA en_US 2007-01-11T11:56:23Z en_US 2007-06-07T10:04:04Z 2007-01-11T11:56:23Z en_US 2007-06-07T10:04:04Z 2000-07 en_US
dc.identifier.citation Berger, DK et al.2000. Bean polygalacturonase inhibitor protein-1 (PGIP-1) inhibits. Physiological and molecular plant pathology, vol 57(1), pp 5 - 14 en_US
dc.identifier.issn 0885-5765 en_US
dc.identifier.uri en_US
dc.description.abstract Stenocarpella maydis, a fungal pathogen of maize, produced polygalacturonases (PGs) when grown on pectin or maize cell walls. An extract from bean (Phaseolus vulgaris L.) which contained an active inhibitor of Aspergillus niger PG, also inhibited S. maydis PG in a reducing sugar assay. Bean polygalacturonase inhibitor protein (PGIP) was purified from the extract by affinity chromatography, and this inhibited the S. maydis PG in reducing sugar assays and an overlay gel activity; assay. Inhibition was abolished by boiling of the PGIP. Since purified PGIP could still be a mixture of PGIPs with similar physical properties but different inhibitory activities on different fungal PGs, the bean pgip-1 gene was cloned and expressed in transgenic tomato, PGIP extracts from the transgenic tomato inhibited both A. niger and S. maydis PG, whereas extracts from a control untransformed tomato did not. This indicated that bean PGIP-1 is able to inhibit the S. maydis PGs. en_US
dc.format.extent 234893 bytes en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en en_US
dc.publisher Academic press Ltd en_US
dc.rights Copyright: 2000 Academic Press. en_US
dc.subject Stenocarpella maydis en_US
dc.subject Phaseolus Vulgaris en_US
dc.subject Polygalacturonase en_US
dc.subject Bean PGIP en_US
dc.subject Plant sciences en_US
dc.title Bean polygalacturonase inhibitor protein-1 (PGIP-1) inhibits polygalacturonases from Stenocarpella maydis en_US
dc.type Article en_US

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