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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/1382

Title: Bean polygalacturonase inhibitor protein-1 (PGIP-1) inhibits polygalacturonases from Stenocarpella maydis
Authors: Berger, DK
Oelofse, D
Arendse, MS
Du Plessis, E
Dubery, IA
Keywords: Stenocarpella maydis
Phaseolus Vulgaris
Plant sciences
Issue Date: Jul-2000
Publisher: Academic press Ltd
Citation: Berger, DK et al.2000. Bean polygalacturonase inhibitor protein-1 (PGIP-1) inhibits. Physiological and molecular plant pathology, vol 57(1), pp 5 - 14
Abstract: Stenocarpella maydis, a fungal pathogen of maize, produced polygalacturonases (PGs) when grown on pectin or maize cell walls. An extract from bean (Phaseolus vulgaris L.) which contained an active inhibitor of Aspergillus niger PG, also inhibited S. maydis PG in a reducing sugar assay. Bean polygalacturonase inhibitor protein (PGIP) was purified from the extract by affinity chromatography, and this inhibited the S. maydis PG in reducing sugar assays and an overlay gel activity; assay. Inhibition was abolished by boiling of the PGIP. Since purified PGIP could still be a mixture of PGIPs with similar physical properties but different inhibitory activities on different fungal PGs, the bean pgip-1 gene was cloned and expressed in transgenic tomato, PGIP extracts from the transgenic tomato inhibited both A. niger and S. maydis PG, whereas extracts from a control untransformed tomato did not. This indicated that bean PGIP-1 is able to inhibit the S. maydis PGs.
URI: http://hdl.handle.net/10204/1382
ISSN: 0885-5765
Appears in Collections:Plant biotechnology
General science, engineering & technology

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