ResearchSpace
→
Browsing by Author

Browsing by Author "Visser, Daniel F"

Now showing items 1-10 of 10

Application of biocatalysis in synthetic chemistry

Author: Brady, D ; Molefe, M ; Steenkamp, Lucia H ; Parkinson, C ; Jordaan, J ; Visser, Daniel F ; Simpson, C ; Chhiba, Varsha P ; Mathiba, K Date: Nov 2006 It was found that the nitrile hydratases in microorganisms isolated from around the world had infra-species amino acid sequence differences, and this provided an explanation for the variability of nitrile substrate usage within the species. ... Read more
Biocatalytic preparation of 5-methyluridine (5-MU)

Author: Gordon, GER ; Visser, Daniel F ; Bode, ML ; Brady, D Date: Nov 2008 The potential of chemo-enzymatic methods to produce active pharmaceutical ingredients (APIs) such as stavudine (d4T) and zidovudine (AZT) has been demostrated during this investigation. The effect of increasing reactor productivity to ... Read more
Characterisation of two bifunctional cellulase-xylanase enzymes isolated from a bovine rumen metagenome library

Author: Rashamuse, KJ ; Visser, Daniel F ; Hennessy, F ; Kemp, J ; Van der Merwe, MP ; Badenhorst, J ; Ronneburg, T ; Francis-Pope, R ; Brady, D Date: Feb 2013 Ruminant digestive tract microbes hydrolyse plant biomass, and the application of metagenomic techniques can provide good coverage of their glycosyl hydrolase enzymes. A metagenomic library of circa 70,000 fosmids was constructed from bacterial ... Read more
Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36

Author: Visser, Daniel F ; Hennessy, F ; Rashamuse, KJ ; Louw, ME ; Brady, D Date: Mar 2010 A purine nucleoside phosphorylase from the alkaliphile Bacillus halodurans Alk36 has been cloned and over expressed in E. coli. The purified enzyme had a kcat of 2.03 x 10-9 s-1 and a km of 206 µM on guanosine. The optimal pH range was between ... Read more
Developing South Africa's biomanufacturing industry through a hub for open innovation: Experiences from the Biomanufacturing Industry Development Centre

Author: Visser, Daniel F Date: Oct 2017 This presentation showcases the Biomanufacturing Industry Development Centre, and discusses how an open innovation hub can contribute towards growth in the South African biomanufacturing industry. Read more
Enzymatic synthesis of 5-methyluridine by transglycosylation of guanosine and thymine

Author: Visser, Daniel F ; Gordon, GER ; Bode, ML ; Mathiba, K ; Brady, D Date: May 2012 5-Methyluridine (5-MU) is an intermediate in the synthesis of ß-thymidine and the antiretroviral drugs stavudine (d4T) and zidovudine (AZT)1-3. The enzymatic preparation of 5-MU involves transglycosylation4-6 and avoids the formation of ... Read more
Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation

Author: Visser, Daniel F ; Hennessy, F ; Chhiba, Varsha P ; Lakay, F ; van Heerden, E ; Piater, L ; Moller, C ; Litthauer, D ; Brady, D Date: Oct 2006 Isolating novel enzymes to enhance a commercial process is an area of active research. Biosciences Discovery Biology have established screening methods to isolate genes for novel enzymes from extreme environments. Samples were obtained from ... Read more
An integrated chemo-enzymatic route for preparation of ß-thymidine, a key intermediate in the preparation of antiretrovirals

Author: Gordon, GER ; Bode, ML ; Visser, Daniel F ; Lepuru, MJ ; Zeevaart, JG ; Ragubeer, N ; Ratsaka, M ; Walwyn, DR ; Brady, D Date: Jan 2011 A chemo-enzymatic method for production of ß-thymidine, an intermediate in the synthesis of antiretrovirals, is described. Guanosine and thymine were converted by means of enzymatic transglycosylation to yield 5-methyluridine (5-MU), which ... Read more
Stabilization of Escherichia coli uridine phosphorylase by evolution and immobilization

Author: Visser, Daniel F ; Hennessy F ; Rashamuse J ; Pletschke B ; Brady D Date: Aug 2010 Uridine phosphorylase from Escherichia coli was evolved by iterative saturation mutagenesis. The best mutant showed a temperature optimum of 60C and a half-life of 17.3 h at 60C. The mutant enzyme, as well as a purine nucleoside phosphorylase ... Read more

Now showing items 1-10 of 10

Browse

All of ResearchSpace

Quick Links

Legislation and compliance

General Enquiries

Tel: + 27 12 841 2911
Email: callcentre@csir.co.za

Physical Address
Meiring Naudé Road
Brummeria
Pretoria
South Africa

Postal Address
PO Box 395
Pretoria 0001
South Africa

Social Connect

Resources on this site are free to download and reuse according to associated licensing provision. Please read the terms and conditions of usage of each resource.

Browsing by Author "Visser, Daniel F"

Browsing by Author "Visser, Daniel F"

Browse

All of ResearchSpace

Quick Links

Legislation and compliance

General Enquiries

Social Connect