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Femtosecond laser assisted photo-transfection and differentiation of mouse embryonic stem cells

Author: Thobakgale, Setumo L ; Manoto, Sello L ; Ombinda-Lemboumba, Saturnin ; Maaza, M ; Mthunzi-Kufa, Patience Date: Jan 2018 In tissue engineering research, stem cells have been used as starting material in the synthesis of mammalian cells for the treatment of various cell based diseases. This is done by manipulating the DNA content of the cells to induce a specific ... Read more
Femtosecond laser surface structuring of molybdenum thin films

Author: Kotsedi, L ; Mthunzi-Kufa, Patience ; Nuru, ZY ; Eaton, SM ; Sechoghela, P ; Mongwaketsi, N ; Ramponi, R ; Maaza, M Date: Oct 2015 This contribution reports on the femtosecond surface structuring of molybdenum thin coatings deposited by electron beam evaporation onto Corning glass substrates. The 1-D type periodic grating lines created by such an ablation showed that the ... Read more
Generating shaped femtosecond pulses in the far infrared using a spatial light modulator and difference frequency generation

Author: Botha, N ; Botha, L ; Du Plessis, A ; Schwoerer, H Date: 31 Aug 2010 Femtosecond pulse shaping can be done by different kinds of pulse shapers, such as liquid crystal spatial light modulators (LC SLM), acousto optic modulators (AOM) and deformable and movable mirrors. A few applications where pulse shaping is ... Read more
Near infrared femtosecond laser-induced bacterial inactivation

Author: Maphanga, Charles P ; Manoto, Sello L ; Ombinda-Lemboumba, Saturnin ; Osualale, O ; Mthunzi-Kufa, Patience Date: Mar 2019 The use of light to inactivate microbes as an alternative method to the traditional methods of controlling microorganisms continues to draw the attention of researchers. Traditional methods of sterilization and/or pasteurization using chemicals ... Read more
Photo-transfection and selective optical differentiation of embryonic stem cells

Author: Mthunzi-Kufa, Patience ; Gunn-Moore, F ; Dholakia, K Date: Sep 2010 Due to their self-renewal and pluripotency characteristics, stem cells possess the potential to dramatically advance current therapies in tissue regeneration and engineering. In this work, we report for the first time that femtosecond laser ... Read more
Phototransfection of mammalian cells using femtosecond laser pulses: optimization and applicability to stem cell differentiation

Author: Mthunzi-Kufa, Patience ; Dholakia, K ; Gunn-Moore, F Date: Jul 2010 Recently, femtosecond laser pulses have been utilized for the targeted introduction of genetic matter into mammalian cells. This rapidly expanding and developing novel optical technique using a tightly focused laser light beam is called ... Read more
Phototransfection of mouse embryonic stem cells with plasmid DNA using femtosecond laser pulses

Author: Thobakgale, Setumo L ; Manoto, Sello L ; Ombinda-Lemboumba, Saturnin ; Maaza, M ; Mthunzi-Kufa, Patience Date: Feb 2017 Cellular manipulation by delivery of molecules into cells has been applied extensively in tissue engineering research for medical applications. The different molecular delivery techniques used range from viral and chemical agents to physical ... Read more

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Browsing by Subject "Femtosecond"

Browsing by Subject "Femtosecond"

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