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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/5189

Title: Phototransfection of mammalian cells using femtosecond laser pulses: optimization and applicability to stem cell differentiation
Authors: Mthunzi, P
Dholakia, K
Gunn-Moore, F
Keywords: Phototransfection
Femtosecond
Laser pulses
Cell arresting
Neuroblastomas
Pluripotent stem cells
Embryonic stem cell differentiation
Biomedical optics
Mammalian cells
Issue Date: Jul-2010
Publisher: Society of Photo-optical Instrumentation Engineers (SPIE)
Citation: Mthunzi, P, Dholakia, K and Gunn-Moore, F. 2010. Phototransfection of mammalian cells using femtosecond laser pulses: optimization and applicability to stem cell differentiation. Journal of Biomedical Optics, Vol 15(4), pp 041507
Series/Report no.: Workflow request;6084
Abstract: Recently, femtosecond laser pulses have been utilized for the targeted introduction of genetic matter into mammalian cells. This rapidly expanding and developing novel optical technique using a tightly focused laser light beam is called phototransfection. Extending previous studies, we show that femtosecond lasers can be used to phototransfect a range of different cell lines, and specifically that this novel technology can also transfect mouse embryonic stem cell colonies with 25% efficiency. Notably, we show the ability of differentiating these cells into the extraembryonic endoderm using phototransfection. Furthermore, we present two new findings aimed at optimizing the phototransfection method and improving applicability: first, the influence of the cell passage number on the transfection efficiency is explored and, second, the ability to enhance the transfection efficiency via whole culture treatments. Our results should encourage wider uptake of this methodology
Description: Copright: 2010 Society of Photo-optical Instrumentation Engineers (SPIE)
URI: http://hdl.handle.net/10204/5189
ISSN: 1083-3668
Appears in Collections:National Centre for nano-structured materials
Nanotechnology
Laser physics and technology
General science, engineering & technology

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