Author:Mathomu, LM; Myer, MS; Kenyon, CPDate:01 Sep 2010Efforts are underway to produce transgenic pigs as source of organ transplants, transgenic fish for food, and transgenic livestock that are resistant to many animal diseases and can survive harsh environmental conditions. Nguni breed of cattle ...Read more
Author:Frederick, J; Brady, D; Dirr, HDate:Oct 2006A versatile nitrile-degrading bacterium, Rhodococcus rhodochrous ATCC BAA-870, was isolated through enrichment culturing of soil samples from Johannesburg, South Africa. Useful nitrile-metabolising activity of a potential biocatalyst, Rhodococcus ...Read more
Author:Dudas, J; Hanika, JDate:Jan 2009The hydrogenation of thymol and the racemisation of a menthol mixture were investigated as part of the development of novel process for the production of l-menthol. The key innovation in this l-menthol process is the enzymatic resolution of ...Read more
Author:Steenkamp, Lucia HDate:01 Sep 2010The main objective of the development of a two-step “green” synthesis for (-)-ambafuran production is to find an alternative synthesis of (-) Ambrox from sclareol, to use a bioconversion or biocatalysis route, and that it results in a natural ...Read more
Author:Visser, Daniel F; Gordon, GER; Bode, ML; Mathiba, K; Brady, DDate:May 20125-Methyluridine (5-MU) is an intermediate in the synthesis of ß-thymidine and the antiretroviral drugs stavudine (d4T) and zidovudine (AZT)1-3. The enzymatic preparation of 5-MU involves transglycosylation4-6 and avoids the formation of ...Read more
Author:Mbanjwa, M; Chen, H; Fourie, L; Ngwenya, S; Land, KDate:Jun 2014Multiplexed or parallelised droplet microfluidic systems allow for increased throughput in the production of emulsions and microparticles, while maintaining a small footprint and utilising minimal ancillary equipment. The current paper ...Read more
Author:Strong, PJ; Burgess, JEDate:Sep 2008The aim of this work was to characterise wine-related wastewaters and ascertain the wastewater concentrations that were optimal for treatment by Trametes pubescens. Laccase production was also monitored. Crudely purified laccase was tested ...Read more
Author:Potgieter, S; Land, K; Kotze, L; Sparrow, RDate:2010Microfluidics is a multi-disciplinary field that deals with the behaviour, control and manipulation of fluids constrained to sub-millilitre volumes. It is proving to be a useful tool for biological studies, affording advantages such as reduced ...Read more
Author:Rashamuse, KJ; Burton, SG; Stanfford, WHL; Cowan, DADate:Feb 2007An esterase producing Burkholderia multivorans UWC10 strain was isolated by culture enrichment. A shotgun library of B. multivorans UWC10 genomic DNA was screened for esterase activity and a recombinant clone conferring an esterolytic phenotype ...Read more
Author:Steenkamp, Lucia H; Brady, DDate:Dec 2008Although the enantioselective kinetic resolution of ester racemates of the non-steroidal antiinflammatory drug naproxen ([2-(6-methoxy-2-naphthyl) propionic acid]) is a common demonstration for biocatalysis, the enantiomeric excess of the ...Read more
Author:De Koning, AJDate:Jul 2004Anchovy were stored at 17, 8, 0 and -6 degrees C. Samples were withdrawn periodically, the lipids extracted, purified, and analysed for free and esterified cholesterol. From the results of these tests the rates of the enzymatic esterification ...Read more
Author:Ramchuran, Santosh O; Berger, E; Du Plessis, E; Crampton, BG; Louw, MEDate:Sep 2006In order to investigate the possibility of using the FliC display system for biotransformation in B. halodurans, construct pSECNLipC habouring the LipA gene from Geobacillus thermoleovorans was designed and evaluated in shake flasks and batch ...Read more
Author:Kenyon, CP; Steyn, A; Roth, Robyn L; Steenkamp, PA; Nkosi, TC; Oldfield, LCDate:Jul 2011Kinase and synthetase enzymes utilise C8D-ATP in preference to non-deuterated ATP. The KIE obtained at low ATP concentrations is clearly a primary KIE demonstrating strong evidence that the bond to the isotopically substituted hydrogen is ...Read more
Author:Steenkamp, Lucia H; Brady, DDate:03 Mar 2003This study focused on the identification of suitable lipase or esterase activity for enantiomeric resolution of (R, S)-naproxen. For an economically viable reaction the enantiomeric ratio (E) should preferably be >100, while maximising the ...Read more
Author:Brady, D; Jordaan, J; Simpson, C; Chetty, A; Arumugam, C; Moolman, FSDate:31 Jan 2008Enzymes have found extensive and growing application in the field of chemical organic synthesis and resolution of chiral intermediates. In order to stabilise the enzymes and to facilitate their recovery and recycle, they are frequently ...Read more