Author:Bumunang, EW; Babalola, OO; Barros, EDate:2013This study examined the impact of genetically modified corn on bacterial functional community in the rhizosphere. Rhizospheric soil samples from GM and non-GM corn were collected at 30 days after sowing (DAS) and at post-harvest from two ...Read more
Author:Steyn, Maronel; Genthe, BettinaDate:May 2009Much attention is given to the recording of diarrhoea incidence among children under the age of 5. This is used as an indicator of health of the community since diarrhoea historically has a more profound effect on children and elderly people. ...Read more
Author:Mnisi, SM; Louw, M; Theron, JDate:Apr 2005A genomic library of Bacillus coagulans strain 81 -11 was screened in Escherichia coli JM83 for lipolytic activity by using tributyrin agar plates. A 2.4 kb DNA fragment was subcloned from a lipolytic-positive clone and completely sequenced. ...Read more
Author:Lalloo, Rajesh; Moonsamy, Ghaneshree; Ramchuran, Santosh O; Görgens, J; Gardiner, NDate:2010When B. cereus was tested in plate well inhibition studies, no production of antimicrobial compounds was detected. B. cereus had a high growth rate (0.96.h−1), whereas Aer. hydrophila concentration decreased by ∼70% in co-culture experiments. ...Read more
Author:Rabie, CJ; Lubben, A; Marais, GJ; Jansen van Vuuren, HDate:01 Apr 1997Estimation of fungal contamination of barley grain is important as certain fungi can proliferate during the malting process. The following factors which may affect the enumeration of fungi were evaluated: dilution versus direct plating, ...Read more
Author:Komakech Otim, CDate:Dec 2008A study was undertaken to compare growth potential of three Eucalyptus species of natural origin in South Eastern Australia, with commercially grown species on mid and high altitude temperate sites in the summer rainfall growing zones in South ...Read more
Author:Berger, E; Du Plessis, E; Gerber, I; Crampton, Michael C; Nxumalo, NP; Louw, MDate:Jan 2009The flagellin type III secretion pathway of Bacillus halodurans BhFC01 (-hag) was modified by the inactivation of fliD. An in-frame flagellin gene fusion polypeptide construct was expressed, and the heterologous peptides were secreted as ...Read more
Author:Thuku, RN; Brady, D; Benedik, MJ; Sewell, BTDate:Mar 2009The nitrilases are enzymes that convert nitriles to the corresponding acid and ammonia. They are members of a superfamily, which includes amidases and occur in both prokaryotes and eukaryotes. The superfamily is characterized by having a ...Read more
Author:Gcebe, N; Rutten, V; Gey van Pittius, N; Naicker, Brendon; Michel, ADate:Apr 2017Non-tuberculous mycobacteria (NTM) are ubiquitous in the environment, and an increasing number of NTM species have been isolated and characterized from both humans and animals, highlighting the zoonotic potential of these bacteria. Host ...Read more
Author:Rashamuse, KJ; Burton, SG; Cowan, DADate:Nov 2007Isolation and identification of bacterial isolates with specific ferulic acid (FA) esterase activity and cloning of a gene encoding activity. A micro-organism with ethyl ferulate hydrolysing (EFH) activity was isolated by culture enrichment ...Read more
Author:Moolman, FS; Cloete, TE; Thantsha, MSDate:Nov 2005Prebiotics is a non-digestible food ingredient that beneficially affects the host by selectively stimulating the growth and/or activity of one or a limited number of bacteria in the colon (Roberfroid, 1998; Theuer et al., 1998; Young, 1998; ...Read more
Author:Thantsha, MS; Cloete, TE; Moolman, FS; Labuschagne, Philip WDate:Jan 2009Gastric acidity is the main factor affecting viability of probiotics in the gastrointestinal tract (GIT). This study investigated the survival in simulated gastrointestinal fluids of Bifidobacterium longum Bb-46 encapsulated in interpolymer ...Read more
Author:Le Roux, Wouter J; Van Blerk, GNDate:Oct 2011A real-time polymerase chain reaction (PCR) assay utilizing high resolution melt (HRM) curve analysis was developed and tested for the monitoring of Vibrio cholerae in water samples. The assay utilized previously published primers that are ...Read more