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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/1550

Title: Enumeration of fungi in barley
Authors: Rabie, CJ
Lubben, A
Marais, GJ
Jansen van Vuuren, H
Keywords: Barley
Microbiology
Fungal contamination
Issue Date: 1-Apr-1997
Publisher: Elsevier Science BV
Citation: Rabie, CJ, et al. 1997. Enumeration of fungi in barley. International Journal of Food Microbiology, vol. 35(2), pp 117-127
Abstract: Estimation of fungal contamination of barley grain is important as certain fungi can proliferate during the malting process. The following factors which may affect the enumeration of fungi were evaluated: dilution versus direct plating, pre-soaked versus unsoaked grain, five culture media: potato dextrose agar (PDA), acidified Czapek-Dox agar (ACA), pentachloronitrobenzene agar; (PCNB) dichloran rose bengal chloramphenicol agar (DRBC) and malt salt agar; two disinfectants' ethanol/water (80:20 v/v) and sodium hypochlorite (3.5% w/v in H2O). Two barley samples, one having a high incidence of storage fungi and one with a high incidence of field fungi were used and most fungi were identified to species level. Results showed that direct plating was superior to dilution plating for assessing the mycoflora of barley. Unsoaked grain gave significantly higher counts than pre-soaked grain in the case of Alternaria alternata, Rhizopus oryzae, Epicoccum nigrum and Mucor spp. Pre-soaked grain resulted in higher counts of Penicillium spp. Chlorine disinfection resulted in significantly higher counts of Aspergillus flavus, Eurotium spp. and Penicillium spp. Ethanol disinfection resulted in higher counts of Mucor spp., Phoma sorghina, Rhizopus oryzae and Aspergillus restrictus. PDA and ACA, in general gave some what better results than DRBC for both field and storage fungi. PCNB consistently gave the highest Fusarium counts. More than thirty fungal genera were found in the two samples.
URI: http://hdl.handle.net/10204/1550
http://hdl.handle.net/10204/1550
ISSN: 0168-1605
Appears in Collections:General science, engineering & technology
Agroprocessing and chemical technology

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