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Please use this identifier to cite or link to this item:
http://hdl.handle.net/10204/3314
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| Title: | Inactivation of genes encoding extracellular proteases in bacillus halodurans BhFC01 and the impact on its modified flagellin type III secretion pathway towards improving peptide expression |
| Authors: | Berger, E
Du Plessis, E
Gerber, I
Crampton, MC
Nxumalo, NP
Louw, M |
| Keywords: | Bacillus halodurans
Gene encoding
Extracellular proteases
Flagellin-fusion
Modified flagellin
Peptide expression
Microbiology |
| Issue Date: | Jan-2009 |
| Publisher: | American Society for Microbiology |
| Citation: | Berger, E, Du Plessis, E; Gerber, I et al. 2009. Inactivation of genes encoding extracellular proteases in bacillus halodurans BhFC01 and the impact on its modified flagellin type III secretion pathway towards improving peptide expression. Applied and Environmental Microbiology, Vol. 75(1), pp 1-23 |
| Abstract: | The flagellin type III secretion pathway of Bacillus halodurans BhFC01 (-hag) was modified by the inactivation of fliD. An in-frame flagellin gene fusion polypeptide construct was expressed, and the heterologous peptides were secreted as flagellin fusion monomers. The stability of the secreted monomers was significantly enhanced through gene-targeted inactivation of extracellular proteases |
| Description: | Copyright: 2009 American Society for Microbiology |
| URI: | http://hdl.handle.net/10204/3314 |
| ISSN: | 0099-2240 |
| Appears in Collections: | Microbial expression systems
General science, engineering & technology
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