DSpace
 

Researchspace >
General science, engineering & technology >
General science, engineering & technology >
General science, engineering & technology >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/3314

Title: Inactivation of genes encoding extracellular proteases in bacillus halodurans BhFC01 and the impact on its modified flagellin type III secretion pathway towards improving peptide expression
Authors: Berger, E
Du Plessis, E
Gerber, I
Crampton, MC
Nxumalo, NP
Louw, M
Keywords: Bacillus halodurans
Gene encoding
Extracellular proteases
Flagellin-fusion
Modified flagellin
Peptide expression
Microbiology
Issue Date: Jan-2009
Publisher: American Society for Microbiology
Citation: Berger, E, Du Plessis, E; Gerber, I et al. 2009. Inactivation of genes encoding extracellular proteases in bacillus halodurans BhFC01 and the impact on its modified flagellin type III secretion pathway towards improving peptide expression. Applied and Environmental Microbiology, Vol. 75(1), pp 1-23
Abstract: The flagellin type III secretion pathway of Bacillus halodurans BhFC01 (-hag) was modified by the inactivation of fliD. An in-frame flagellin gene fusion polypeptide construct was expressed, and the heterologous peptides were secreted as flagellin fusion monomers. The stability of the secreted monomers was significantly enhanced through gene-targeted inactivation of extracellular proteases
Description: Copyright: 2009 American Society for Microbiology
URI: http://hdl.handle.net/10204/3314
ISSN: 0099-2240
Appears in Collections:Microbial expression systems
General science, engineering & technology

Files in This Item:

File Description SizeFormat
Berger_2009.pdf2.59 MBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback