Author:Visser, Daniel F; Hennessy, F; Rashamuse, KJ; Louw, ME; Brady, DDate:Mar 2010A purine nucleoside phosphorylase from the alkaliphile Bacillus halodurans Alk36 has been cloned and over expressed in E. coli. The purified enzyme had a kcat of 2.03 x 10-9 s-1 and a km of 206 µM on guanosine. The optimal pH range was between ...Read more
Author:Crampton, Michael C; Berger, E; Reid, S; Louw, MDate:Jun 2007This study relates to the development of an alkaliphilic, thermotolerant, Gram-positive isolate, Bacillus halodurans Alk36, for the over-production and surface display of chimeric gene products. This bacterium continuously over-produces ...Read more
Author:Du Plessis, EM; Theron, J; Berger, E; Louw, MEDate:Nov 2007A phosphatase secreted by Staphylococcus aureus strain 154 has previously been characterized and classified as a new member of the bacterial class C family of non-specific acid phosphatases. As the acid phosphatase activity can be easily ...Read more
Author:Berger, E; Crampton, Michael C; Nxumalo, NP; Louw, MEDate:Aug 2011Through modification of the flagellin type III secretion pathway of Bacillus halodurans heterologous peptides could be secreted into the medium as flagellin fusion monomers. The stability of the secreted monomers was significantly enhanced ...Read more
Author:Berger, E; Du Plessis, E; Gerber, I; Crampton, Michael C; Nxumalo, NP; Louw, MDate:Jan 2009The flagellin type III secretion pathway of Bacillus halodurans BhFC01 (-hag) was modified by the inactivation of fliD. An in-frame flagellin gene fusion polypeptide construct was expressed, and the heterologous peptides were secreted as ...Read more
Author:Ramchuran, Santosh ODate:Jul 2006A gene encoding a lipolytic enzyme amplified from the alkaliphilic bacterium Bacillus halodurans LBB2 was cloned into the pPICZaB vector and integrated into the genome of the protease deficient yeast strain Pichia pastoris SMD1168H. This ...Read more
Author:Crampton, Michael CDate:Jul 2010This presentation focused on the transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans. It concludes to a successful implementation of a high throughput mRNA sandwich hybridisation ...Read more