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Please use this identifier to cite or link to this item:
http://hdl.handle.net/10204/4381
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| Title: | Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans |
| Authors: | Crampton, M |
| Keywords: | Heterologous genes
Bacillus halodurans
Genetics
Microorganisms
Industrial microorganisms
Genetics of Industrial Microorganisms (GIM 2010) |
| Issue Date: | Jul-2010 |
| Publisher: | CSIR |
| Citation: | Crampton, M. 2010. Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans. 11th International Symposium of the Genetics of Industrial Microorganisms, Melbourne, Australia, June 28- July 1 2010, pp 18 |
| Abstract: | This presentation focused on the transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans. It concludes to a successful implementation of a high throughput mRNA sandwich hybridisation quantification assay. It also found that B. halodurans sD promoter regulated in a similar fashion in complex media as B. subtilis, that vector choice influences transcript levels, that flgM-null mutant improves transcript levels but not protein levels and that peptide production follows transcript profile for LB. |
| Description: | 11th International Symposium of the Genetics of Industrial Microorganisms, Melbourne, Australia, June 28- July 1 2010 |
| URI: | http://hdl.handle.net/10204/4381 |
| Appears in Collections: | Microbial expression systems
General science, engineering & technology
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