Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans

Abstract

This presentation focused on the transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans. It concludes to a successful implementation of a high throughput mRNA sandwich hybridisation quantification assay. It also found that B. halodurans sD promoter regulated in a similar fashion in complex media as B. subtilis, that vector choice influences transcript levels, that flgM-null mutant improves transcript levels but not protein levels and that peptide production follows transcript profile for LB.