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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/4381

Title: Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans
Authors: Crampton, M
Keywords: Heterologous genes
Bacillus halodurans
Genetics
Microorganisms
Industrial microorganisms
Genetics of Industrial Microorganisms (GIM 2010)
Issue Date: Jul-2010
Publisher: CSIR
Citation: Crampton, M. 2010. Transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans. 11th International Symposium of the Genetics of Industrial Microorganisms, Melbourne, Australia, June 28- July 1 2010, pp 18
Abstract: This presentation focused on the transcriptional analysis of heterologous gene expression using the endogenous sD promoter from Bacillus halodurans. It concludes to a successful implementation of a high throughput mRNA sandwich hybridisation quantification assay. It also found that B. halodurans sD promoter regulated in a similar fashion in complex media as B. subtilis, that vector choice influences transcript levels, that flgM-null mutant improves transcript levels but not protein levels and that peptide production follows transcript profile for LB.
Description: 11th International Symposium of the Genetics of Industrial Microorganisms, Melbourne, Australia, June 28- July 1 2010
URI: http://hdl.handle.net/10204/4381
Appears in Collections:Microbial expression systems
General science, engineering & technology

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