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dc.contributor.author Madala, NE
dc.contributor.author Steenkamp, PA
dc.contributor.author Piater, LA
dc.contributor.author Dubery, IA
dc.date.accessioned 2014-04-29T06:55:47Z
dc.date.available 2014-04-29T06:55:47Z
dc.date.issued 2013-10
dc.identifier.citation Madala, N.E, Steenkamp, P.A, Piater, L.A and Dubery, I.A. 2013. Metabolomic analysis of isonitrosoacetophenone-induced perturbations in phenolic metabolism of Nicotiana tabacum cells. Phytochemistry, vol. 94, pp 82-90 en_US
dc.identifier.issn 0031-9422
dc.identifier.uri http://ac.els-cdn.com/S0031942213001921/1-s2.0-S0031942213001921-main.pdf?_tid=da81eb46-cc60-11e3-8edb-00000aacb360&acdnat=1398420390_f9f68238dc74da19c7115cbdab79cd37
dc.identifier.uri http://hdl.handle.net/10204/7378
dc.description Copyright: 2014 Elsevier. This is AN ABSTRACT ONLY. The definitive version is published in Phytochemistry, vol. 94, pp 82-90 en_US
dc.description.abstract Plants have developed biochemical and molecular responses to adapt to different stress environments. One of the characteristics of the multi-component defence response is the production of defence-related metabolites. Plant defences can be triggered by various stimuli, including synthetic or naturally occurring molecules, especially those derived from pathogens. In the current study, Nicotiana tabacum cell suspensions were treated with isonitrosoacetophenone (INAP), a subcomponent of a plant-derived stress metabolite with anti-fungal and anti-oxidant properties, in order to investigate the effect thereof on cellular metabolism. Subsequent metabolomic-based analyses were employed to evaluate changes in the metabolome. UPLC-MS in conjunction with multivariate data analyses was found to be an appropriate approach to study the effect of chemical inducers like INAP on plant metabolism in this model system. Principal component analysis (PCA) indicated that INAP is capable of inducing time-dependent metabolic perturbations in the cultured cells. Orthogonal projection to latent structures discriminant analysis (OPLS-DA) revealed metabolites of which the levels are affected by INAP, and eight of these were tentatively annotated from the mass spectral data and online databases. These metabolites are known in the context of plant stress- and defence responses and include benzoic- or cinnamic acid derivatives that are either glycosylated or quinilated as well as flavonoid derivatives. The results indicate that INAP affects the shikimate-, phenylpropanoid- and flavonoid pathways, the products of which may subsequently lead to an anti-oxidant environment in vivo. en_US
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.relation.ispartofseries Workflow;12430
dc.subject 2-Keto-2-phenyl-acetaldoxime en_US
dc.subject Biotransformation en_US
dc.subject Induced-defences en_US
dc.subject Isonitrosoacetophenone en_US
dc.subject Metabolism en_US
dc.subject Metabolomics en_US
dc.subject Multivariate statistics en_US
dc.subject Nicotiana tabacum en_US
dc.subject Xenobiotics en_US
dc.title Metabolomic analysis of isonitrosoacetophenone-induced perturbations in phenolic metabolism of Nicotiana tabacum cells en_US
dc.type Article en_US


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