ResearchSpace

Gelatine-based biosensor for molecular screening of aspirin and paracetamol via surface enhanced Raman spectroscopy

Show simple item record

dc.contributor.author Thobakgale, Setumo L
dc.contributor.author Manoto, Sello L
dc.contributor.author Ombinda- Lemboumba, Saturnin
dc.contributor.author Mthunzi-Kufa, Patience
dc.date.accessioned 2021-04-23T16:18:22Z
dc.date.available 2021-04-23T16:18:22Z
dc.date.issued 2020-02
dc.identifier.citation Thobakgale, S.L., Manoto, S.L., Ombinda- Lemboumba, S. & Mthunzi-Kufa, P. 2020. Gelatine-based biosensor for molecular screening of aspirin and paracetamol via surface enhanced Raman spectroscopy. http://hdl.handle.net/10204/12001 . en_ZA
dc.identifier.isbn 9781510633018
dc.identifier.isbn 9781510633025
dc.identifier.issn 1996-756X
dc.identifier.uri Doi: 10.1117/12.2546479
dc.identifier.uri http://hdl.handle.net/10204/12001
dc.description.abstract Polypeptide gelatine has been used extensively in microbiology to enhance cellular adhesion and growth. Likewise, fabrication of biochemical sensors using a variety of organic material and nanomaterials is a growing research area particularly in experiments involving single molecular screening. Both fields of study exploit the various interactions that occur at molecular level such as charge-charge binding, hydrogen bonding and van Der Waals forces. In this work, a thin film gelatine based biosensor, containing amino acids such as glycine, proline and hydroxy-proline was synthesized on glass slides using the self-assembly method. Further -adaption involved coating gold nanoparticles onto the substrate to enhance chemical binding and improve signal intensity and sensitivity. Pharmaceutical drugs aspirin and paracetamol were used as analytes to explore the qualitative and quantitative capabilities of the sensor in molecular screening through surface enhanced Raman spectroscopy (SERS). The results showed a distinguishable qualitative difference between the Raman spectra of gelatine-drug (Gel-D) and gelatine-gold-drug (Gel-Au-D) fabricated sensors. Similarly in both Gel-D and Gel-Au-D, the peak areas of the functional groups found in both aspirin and paracetamol increased with drug concentration, yielding satisfactory calibration curves. The gelatine based biosensor thus holds potential as an in vitro sensing platform for screening of pharmaceutical drugs. en_US
dc.format Abstract en_US
dc.language.iso en en_US
dc.relation.uri https://spie.org/Publications/Proceedings/Paper/10.1117/12.2546479?SSO=1 en_US
dc.relation.uri https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11269/1126905/Gelatine-based-biosensor-for-molecular-screening-of-aspirin-and-paracetamol/10.1117/12.2546479.full en_US
dc.source Proceedings of SPIE 11269, Synthesis and Photonics of Nanoscale Materials XVII, 1126905, San Franscisco Califorinia, 1-4 February 2020 en_US
dc.subject Aspirin en_US
dc.subject Gelatine en_US
dc.subject Nanomaterials en_US
dc.subject Paracetamol en_US
dc.subject Surface enhanced Raman spectroscopy en_US
dc.subject SERS en_US
dc.title Gelatine-based biosensor for molecular screening of aspirin and paracetamol via surface enhanced Raman spectroscopy en_US
dc.type Conference Presentation en_US
dc.description.pages 9 en_US
dc.description.note Copyright: 2020 SPIE. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website: https://spie.org/Publications/Proceedings/Paper/10.1117/12.2546479?SSO=1 untranslated en_US
dc.description.cluster Manufacturing
dc.description.impactarea Bio-photonics en_US
dc.identifier.apacitation Thobakgale, S. L., Manoto, S. L., Ombinda- Lemboumba, S., & Mthunzi-Kufa, P. (2020). Gelatine-based biosensor for molecular screening of aspirin and paracetamol via surface enhanced Raman spectroscopy. http://hdl.handle.net/10204/12001 en_ZA
dc.identifier.chicagocitation Thobakgale, Setumo L, Sello L Manoto, Saturnin Ombinda- Lemboumba, and Patience Mthunzi-Kufa. "Gelatine-based biosensor for molecular screening of aspirin and paracetamol via surface enhanced Raman spectroscopy." <i>Proceedings of SPIE 11269, Synthesis and Photonics of Nanoscale Materials XVII, 1126905, San Franscisco Califorinia, 1-4 February 2020</i> (2020): http://hdl.handle.net/10204/12001 en_ZA
dc.identifier.vancouvercitation Thobakgale SL, Manoto SL, Ombinda- Lemboumba S, Mthunzi-Kufa P, Gelatine-based biosensor for molecular screening of aspirin and paracetamol via surface enhanced Raman spectroscopy; 2020. http://hdl.handle.net/10204/12001 . en_ZA
dc.identifier.ris TY - Conference Presentation AU - Thobakgale, Setumo L AU - Manoto, Sello L AU - Ombinda- Lemboumba, Saturnin AU - Mthunzi-Kufa, Patience AB - Polypeptide gelatine has been used extensively in microbiology to enhance cellular adhesion and growth. Likewise, fabrication of biochemical sensors using a variety of organic material and nanomaterials is a growing research area particularly in experiments involving single molecular screening. Both fields of study exploit the various interactions that occur at molecular level such as charge-charge binding, hydrogen bonding and van Der Waals forces. In this work, a thin film gelatine based biosensor, containing amino acids such as glycine, proline and hydroxy-proline was synthesized on glass slides using the self-assembly method. Further -adaption involved coating gold nanoparticles onto the substrate to enhance chemical binding and improve signal intensity and sensitivity. Pharmaceutical drugs aspirin and paracetamol were used as analytes to explore the qualitative and quantitative capabilities of the sensor in molecular screening through surface enhanced Raman spectroscopy (SERS). The results showed a distinguishable qualitative difference between the Raman spectra of gelatine-drug (Gel-D) and gelatine-gold-drug (Gel-Au-D) fabricated sensors. Similarly in both Gel-D and Gel-Au-D, the peak areas of the functional groups found in both aspirin and paracetamol increased with drug concentration, yielding satisfactory calibration curves. The gelatine based biosensor thus holds potential as an in vitro sensing platform for screening of pharmaceutical drugs. DA - 2020-02 DB - ResearchSpace DP - CSIR J1 - Proceedings of SPIE 11269, Synthesis and Photonics of Nanoscale Materials XVII, 1126905, San Franscisco Califorinia, 1-4 February 2020 KW - Aspirin KW - Gelatine KW - Nanomaterials KW - Paracetamol KW - Surface enhanced Raman spectroscopy KW - SERS LK - https://researchspace.csir.co.za PY - 2020 SM - 9781510633018 SM - 9781510633025 SM - 1996-756X T1 - Gelatine-based biosensor for molecular screening of aspirin and paracetamol via surface enhanced Raman spectroscopy TI - Gelatine-based biosensor for molecular screening of aspirin and paracetamol via surface enhanced Raman spectroscopy UR - http://hdl.handle.net/10204/12001 ER - en_ZA
dc.identifier.worklist 24028 en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record