ResearchSpace

SWATH-MS based proteomic profiling of Pancreatic Ductal Adenocarcinoma tumours reveals the interplay between the extracellular matrix and related intracellular pathways

Show simple item record

dc.contributor.author Nweke, EE
dc.contributor.author Naicker, P
dc.contributor.author Aaron, S
dc.contributor.author Stoychev, Stoyan J
dc.contributor.author Tabb, DL
dc.contributor.author Jones, OJ
dc.contributor.author Smith, MD
dc.contributor.author Candy, GP
dc.date.accessioned 2020-11-02T10:56:49Z
dc.date.available 2020-11-02T10:56:49Z
dc.date.issued 2020-06
dc.identifier.citation Nweke, E.E. (et.al). 2020. SWATH-MS based proteomic profiling of Pancreatic Ductal Adenocarcinoma tumours reveals the interplay between the extracellular matrix and related intracellular pathways. medRxiv, 39pp. en_US
dc.identifier.uri https://doi.org/10.1101/2020.06.04.20116640
dc.identifier.uri https://www.medrxiv.org/content/10.1101/2020.06.04.20116640v1
dc.identifier.uri http://hdl.handle.net/10204/11656
dc.description This publication is made available under a CC-BY-NC-ND 4.0 International license. Copyright holder is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The item is published in a preprint server. en_US
dc.description.abstract Pancreatic cancer accounts for 2.8% of new cancer cases worldwide and is projected to become by 2030 the second leading cause of cancer-related deaths. Patients of African ancestry appear to be at an increased risk for pancreatic ductal adenocarcinoma (PDAC), with worse severity and outcomes. The purpose of this study was to map the proteomic and genomic landscape of a cohort of PDAC patients of African ancestry. Thirty tissues (15 tumours and 15 normal adjacent tissues) were obtained from consenting South African PDAC patients. Optimisation of the sample preparation method allowed for the simultaneous extraction of high-purity protein and DNA for SWATH-MS and OncoArray SNV analyses. We quantified 3402 proteins with 49 upregulated and 35 downregulated proteins at a minimum 2.1 fold change and FDR adjusted p-value (q-value) = 0.01 when comparing tumour to normal adjacent tissue. Many of the upregulated proteins in the tumour samples are involved in extracellular matrix formation (ECM) and related intracellular pathways. Proteins such as EMIL1, ZCCHV and KBTB2 involved in the regulation of ECM proteins were observed to be dysregulated in pancreatic tumours. Approximately 11% of the dysregulated proteins, including ISLR, BP1, PTK7 and OLFL3, were predicted to be secretory proteins. Additionally, we identified missense mutations in some upregulated proteins, such as MYPN, ESTY2 and SERPINB8. These findings help in further elucidating the biology of PDAC and may aid in identifying future plausible markers for the disease. en_US
dc.language.iso en en_US
dc.publisher medRxiv en_US
dc.relation.ispartofseries Worklist;23833
dc.subject Pancreatic cancer en_US
dc.subject Proteins en_US
dc.subject SWATH-MS en_US
dc.subject Extracellular matrix en_US
dc.title SWATH-MS based proteomic profiling of Pancreatic Ductal Adenocarcinoma tumours reveals the interplay between the extracellular matrix and related intracellular pathways en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record