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dc.contributor.author Lategan, KL
dc.contributor.author Walters, Chavon R
dc.contributor.author Pool, EJ
dc.date.accessioned 2019-02-04T06:43:55Z
dc.date.available 2019-02-04T06:43:55Z
dc.date.issued 2019-01
dc.identifier.citation Lategan, K.L., Walters, C.R. and Pool, E.J. 2019. The effects of silver nanoparticles on RAW 264.7. Macrophages and human whole blood cell cultures. Frontiers In Bioscience, Landmark, vol. 24: 347-365 en_US
dc.identifier.issn 1093-9946
dc.identifier.issn 1093-4715
dc.identifier.uri http://www.bioscience.org/2019/v24/af/4722/3.htm
dc.identifier.uri http://www.bioscience.org/2019/v24/af/4722/list.htm
dc.identifier.uri http://hdl.handle.net/10204/10675
dc.description Copyright: 2019 Frontiers in Bioscience. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, please consult the publisher's website. The definitive version can be obtained from: http://www.bioscience.org/2019/v24/af/4722/list.htm en_US
dc.description.abstract Silver nanoparticles (AgNPs) are commonly found in consumer products due to their antimicrobial properties. This study evaluated the effects of AgNPs on the murine macrophage cell line RAW 264.7 and human whole blood cell cultures (WBCs). Effects of AgNPs on RAW cells were assessed in the presence or absence of lipopolysaccharide (LPS). Effects of AgNPs on WBCs were monitored under basal conditions and in the presence of either LPS or phytohaemmagglutinin (PHA). AgNPs were cytotoxic to WBCs at 250 µg/ml. Under basal conditions, RAW cells = 62.5. µg/ml and WBCs > 25 µg/ml AgNPs induced biomarkers associated with inflammation. Under LPS stimulated conditions, 250 µg/ml AgNP inhibited biomarkers associated with inflammation for both cultures. Under basal conditions, and in the presence of 250 µg/ml AgNP, WBCs produced acquired immune system cytokines IL-10 and IFN . IL- 10 synthesis by WBCs was partially inhibited by 250 µg/ml AgNP in the presence of PHA. Proteome profiles of RAW cell supernatants show that AgNPs modulate biomarkers associated with inflammation. WBCs proteome analysis shows modulation of biomarkers associated with anti-inflammatory effects. en_US
dc.language.iso en en_US
dc.publisher Frontiers in Bioscience en_US
dc.relation.ispartofseries Worklist;21382
dc.subject Blood cell cultures en_US
dc.subject Macrophages en_US
dc.subject RAW 264.7. en_US
dc.subject Silver nanoparticles en_US
dc.title The effects of silver nanoparticles on RAW 264.7. Macrophages and human whole blood cell cultures en_US
dc.type Article en_US


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