dc.contributor.author |
Rapheeha, OKL
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dc.contributor.author |
Roux-van der Merwe, MP
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dc.contributor.author |
Badenhorst, J
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dc.contributor.author |
Chhiba, Varsha P
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dc.contributor.author |
Bode, ML
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dc.contributor.author |
Mathiba, Kgama
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dc.contributor.author |
Brady, D
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dc.date.accessioned |
2017-09-01T12:05:13Z |
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dc.date.available |
2017-09-01T12:05:13Z |
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dc.date.issued |
2017-03 |
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dc.identifier.citation |
Rapheeha, O.K.L., Roux-van der Merwe, M.P., Badenhorst, J. et al. 2017. Hydrolysis of nitriles by soil bacteria: variation with soil origin. Journal of Applied Microbiology, vol. 122 (3): 686-697. DOI: 10.1111/jam.13367 |
en_US |
dc.identifier.issn |
1364-5072 |
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dc.identifier.uri |
http://onlinelibrary.wiley.com/doi/10.1111/jam.13367/abstract
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dc.identifier.uri |
DOI: 10.1111/jam.13367
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dc.identifier.uri |
http://hdl.handle.net/10204/9522
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dc.description |
Copyright: 2016 The Society for Applied Microbiology. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, kindly consult the publisher's website. |
en_US |
dc.description.abstract |
The aim of this study was to explore bacterial soil diversity for nitrile biocatalysts, in particular, those for hydrolysis of ß-substituted nitriles, to the corresponding carboxamides and acids that may be incorporated into peptidomimetics. To achieve this, we needed to compare the efficiency of isolation methods and determine the influence of land use and geographical origin of the soil sample. Nitrile-utilizing bacteria were isolated from various soil environments across a 1000 km long transect of South Africa, including agricultural soil, a gold mine tailing dam and uncultivated soil. The substrate profile of these isolates was determined through element-limited growth studies on seven different aliphatic or aromatic nitriles. A subset of these organisms expressing broad substrate ranges was evaluated for their ability to hydrolyse ß-substituted nitriles (3-amino-3-phenylpropionitrile and 3-hydroxy-4-phenoxybutyronitrile) and the active organisms were found to be Rhodococcus erythropolis from uncultivated soil and Rhodococcus rhodochrous from agricultural soils. The capacity for hydrolysis of ß-substituted nitriles appears to reside almost exclusively in Rhodococci. Land use has a much greater effect on the biocatalysis substrate profile than geographical location. Enzymes are typically substrate specific in their catalytic reactions, and this means that a wide diversity of enzymes is required to provide a comprehensive biocatalysis toolbox. This paper shows that the microbial diversity of nitrile hydrolysis activity can be targeted according to land utilization. Nitrile biocatalysis is a green chemical method for the enzymatic production of amides and carboxylic acids that has industrial applications, such as in the synthesis of acrylamide and nicotinamide. The biocatalysts discovered in this study may be applied to the synthesis of peptidomimetics which are an important class of therapeutic compounds. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Wiley |
en_US |
dc.relation.ispartofseries |
Worklist;19228 |
|
dc.subject |
Nitrile biocatalysts |
en_US |
dc.subject |
Soil bacteria |
en_US |
dc.title |
Hydrolysis of nitriles by soil bacteria: variation with soil origin |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
Rapheeha, O., Roux-van der Merwe, M., Badenhorst, J., Chhiba, V. P., Bode, M., Mathiba, K., & Brady, D. (2017). Hydrolysis of nitriles by soil bacteria: variation with soil origin. http://hdl.handle.net/10204/9522 |
en_ZA |
dc.identifier.chicagocitation |
Rapheeha, OKL, MP Roux-van der Merwe, J Badenhorst, Varsha P Chhiba, ML Bode, Kgama Mathiba, and D Brady "Hydrolysis of nitriles by soil bacteria: variation with soil origin." (2017) http://hdl.handle.net/10204/9522 |
en_ZA |
dc.identifier.vancouvercitation |
Rapheeha O, Roux-van der Merwe M, Badenhorst J, Chhiba VP, Bode M, Mathiba K, et al. Hydrolysis of nitriles by soil bacteria: variation with soil origin. 2017; http://hdl.handle.net/10204/9522. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Rapheeha, OKL
AU - Roux-van der Merwe, MP
AU - Badenhorst, J
AU - Chhiba, Varsha P
AU - Bode, ML
AU - Mathiba, Kgama
AU - Brady, D
AB - The aim of this study was to explore bacterial soil diversity for nitrile biocatalysts, in particular, those for hydrolysis of ß-substituted nitriles, to the corresponding carboxamides and acids that may be incorporated into peptidomimetics. To achieve this, we needed to compare the efficiency of isolation methods and determine the influence of land use and geographical origin of the soil sample. Nitrile-utilizing bacteria were isolated from various soil environments across a 1000 km long transect of South Africa, including agricultural soil, a gold mine tailing dam and uncultivated soil. The substrate profile of these isolates was determined through element-limited growth studies on seven different aliphatic or aromatic nitriles. A subset of these organisms expressing broad substrate ranges was evaluated for their ability to hydrolyse ß-substituted nitriles (3-amino-3-phenylpropionitrile and 3-hydroxy-4-phenoxybutyronitrile) and the active organisms were found to be Rhodococcus erythropolis from uncultivated soil and Rhodococcus rhodochrous from agricultural soils. The capacity for hydrolysis of ß-substituted nitriles appears to reside almost exclusively in Rhodococci. Land use has a much greater effect on the biocatalysis substrate profile than geographical location. Enzymes are typically substrate specific in their catalytic reactions, and this means that a wide diversity of enzymes is required to provide a comprehensive biocatalysis toolbox. This paper shows that the microbial diversity of nitrile hydrolysis activity can be targeted according to land utilization. Nitrile biocatalysis is a green chemical method for the enzymatic production of amides and carboxylic acids that has industrial applications, such as in the synthesis of acrylamide and nicotinamide. The biocatalysts discovered in this study may be applied to the synthesis of peptidomimetics which are an important class of therapeutic compounds.
DA - 2017-03
DB - ResearchSpace
DP - CSIR
KW - Nitrile biocatalysts
KW - Soil bacteria
LK - https://researchspace.csir.co.za
PY - 2017
SM - 1364-5072
T1 - Hydrolysis of nitriles by soil bacteria: variation with soil origin
TI - Hydrolysis of nitriles by soil bacteria: variation with soil origin
UR - http://hdl.handle.net/10204/9522
ER -
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en_ZA |