The aim of this article is to investigate the production of soluble cross-reacting material 197 (CRM(sub197)) in Escherichia coli, a safe and effective T-cell-dependent protein carrier for polysaccharides used in the manufacture and application of multivalent conjugate vaccines.
Reference:
Roth, R.L., Van Zyl, P.J., Tsekoa, T.L. et al. 2017. Co-expression of sulphydryl oxidase and protein disulphide isomerase in Escherichia coli allows for production of soluble CRM197. Journal of Applied Microbiology, vol. 122: 1402-1411. DOI: 10.1111/jam.13441
Roth, R. L., Van Zyl, P. J., Tsekoa, T. L., Stoychev, S. H., Mamputha, S., Buthelezi, S. G., & Crampton, M. C. (2017). Co-expression of sulphydryl oxidase and protein disulphide isomerase in Escherichia coli allows for production of soluble CRM197. http://hdl.handle.net/10204/9510
Roth, Robyn L, Petrus J Van Zyl, Tsepo L Tsekoa, Stoyan H Stoychev, Sipho Mamputha, Sindisiwe G Buthelezi, and Michael C Crampton "Co-expression of sulphydryl oxidase and protein disulphide isomerase in Escherichia coli allows for production of soluble CRM197." (2017) http://hdl.handle.net/10204/9510
Roth RL, Van Zyl PJ, Tsekoa TL, Stoychev SH, Mamputha S, Buthelezi SG, et al. Co-expression of sulphydryl oxidase and protein disulphide isomerase in Escherichia coli allows for production of soluble CRM197. 2017; http://hdl.handle.net/10204/9510.
Copyright: 2017 Elsevier. Due to copyright restrictions, the attached PDF file only contains the abstract of the full text item. For access to the full text item, kindly consult the publisher's website.
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