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Proton induced X-ray emission and electron microscopy analysis of induced mutants of sorghum

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dc.contributor.author Mbambo, Z
dc.contributor.author Minnis-Ndimba, R
dc.contributor.author Pineda, C
dc.contributor.author Ndimba, B
dc.contributor.author Bado, S
dc.contributor.author Lin, J
dc.contributor.author Chikwamba, Rachel K
dc.contributor.author Mehlo, L
dc.date.accessioned 2016-02-23T09:13:40Z
dc.date.available 2016-02-23T09:13:40Z
dc.date.issued 2014
dc.identifier.citation Mbambo, Z, Minnis-Ndimba, R, Pineda, C, Ndimba, B, Bado, S,Lin, J, Chikwamba, R and Mehlo, L. Proton induced X-ray emission and electron microscopy analysis of induced mutants of sorghum. Mutagenesis: Exploring Novel Genes and Pathways, Book edited by: Tomlekova, N, Kozgar, I and Wani, R, ISBN: 9789086862344, Publisher: Wageningen Academic Publishers, The Netherlands, Publishing date: July 10, 2014 en_US
dc.identifier.isbn 9789086862344
dc.identifier.uri http://hdl.handle.net/10204/8418
dc.description Permission is granted to archive authors post print version of the book, Publisher: Wageningen Academic Publishers, The Netherlands, Publishing date: July 10, 2014 en_US
dc.description.abstract Gamma irradiation induced alterations in the spatial distribution and localization profiles of Fe, Zn, K, P, Cl, Ca, S, Mn, and Cu in mutant seeds of sorghum were determined using PIXE. The changes included enhanced or diminished accumulation of elements in preferential accumulation tissues and entire changes in cellular localization. Transmission and scanning electron microscopy of the mutants resolved changes in size, shape, ultra-structure and packed cell volumes of protein- and starch bodies. The combined data suggest that induced mutations are an effective tool suitable for simultaneously targeting changes in multiple agronomic and nutritional traits that are crucial for human and animal health in important crops. en_US
dc.language.iso en en_US
dc.publisher Wageningen Academic Publishers en_US
dc.relation.ispartofseries Workflow;11179
dc.subject Proton induced X-ray emission en_US
dc.subject PIXE en_US
dc.subject Novel genes en_US
dc.subject Electron microscopy analysis en_US
dc.subject Micronutrients en_US
dc.subject Protein bodies en_US
dc.subject Starch bodies en_US
dc.subject Gamma irradiation en_US
dc.subject Sorghum en_US
dc.title Proton induced X-ray emission and electron microscopy analysis of induced mutants of sorghum en_US
dc.type Book Chapter en_US
dc.identifier.apacitation Mbambo, Z., Minnis-Ndimba, R., Pineda, C., Ndimba, B., Bado, S., Lin, J., ... Mehlo, L. (2014). Proton induced X-Ray emission and electron microscopy analysis of induced mutants of sorghum., <i>Workflow;11179</i> Wageningen Academic Publishers. http://hdl.handle.net/10204/8418 en_ZA
dc.identifier.chicagocitation Mbambo, Z, R Minnis-Ndimba, C Pineda, B Ndimba, S Bado, J Lin, Rachel K Chikwamba, and L Mehlo. "Proton induced X-ray emission and electron microscopy analysis of induced mutants of sorghum" In <i>WORKFLOW;11179</i>, n.p.: Wageningen Academic Publishers. 2014. http://hdl.handle.net/10204/8418. en_ZA
dc.identifier.vancouvercitation Mbambo Z, Minnis-Ndimba R, Pineda C, Ndimba B, Bado S, Lin J, et al. Proton induced X-ray emission and electron microscopy analysis of induced mutants of sorghum.. Workflow;11179. [place unknown]: Wageningen Academic Publishers; 2014. [cited yyyy month dd]. http://hdl.handle.net/10204/8418. en_ZA
dc.identifier.ris TY - Book Chapter AU - Mbambo, Z AU - Minnis-Ndimba, R AU - Pineda, C AU - Ndimba, B AU - Bado, S AU - Lin, J AU - Chikwamba, Rachel K AU - Mehlo, L AB - Gamma irradiation induced alterations in the spatial distribution and localization profiles of Fe, Zn, K, P, Cl, Ca, S, Mn, and Cu in mutant seeds of sorghum were determined using PIXE. The changes included enhanced or diminished accumulation of elements in preferential accumulation tissues and entire changes in cellular localization. Transmission and scanning electron microscopy of the mutants resolved changes in size, shape, ultra-structure and packed cell volumes of protein- and starch bodies. The combined data suggest that induced mutations are an effective tool suitable for simultaneously targeting changes in multiple agronomic and nutritional traits that are crucial for human and animal health in important crops. DA - 2014 DB - ResearchSpace DP - CSIR KW - Proton induced X-ray emission KW - PIXE KW - Novel genes KW - Electron microscopy analysis KW - Micronutrients KW - Protein bodies KW - Starch bodies KW - Gamma irradiation KW - Sorghum LK - https://researchspace.csir.co.za PY - 2014 SM - 9789086862344 T1 - Proton induced X-ray emission and electron microscopy analysis of induced mutants of sorghum TI - Proton induced X-ray emission and electron microscopy analysis of induced mutants of sorghum UR - http://hdl.handle.net/10204/8418 ER - en_ZA


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