Gamma irradiation induced alterations in the spatial distribution and localization profiles of Fe, Zn, K, P, Cl, Ca, S, Mn, and Cu in mutant seeds of sorghum were determined using PIXE. The changes included enhanced or diminished accumulation of elements in preferential accumulation tissues and entire changes in cellular localization. Transmission and scanning electron microscopy of the mutants resolved changes in size, shape, ultra-structure and packed cell volumes of protein- and starch bodies. The combined data suggest that induced mutations are an effective tool suitable for simultaneously targeting changes in multiple agronomic and nutritional traits that are crucial for human and animal health in important crops.
Reference:
Mbambo, Z, Minnis-Ndimba, R, Pineda, C, Ndimba, B, Bado, S,Lin, J, Chikwamba, R and Mehlo, L. Proton induced X-ray emission and electron microscopy analysis of induced mutants of sorghum. Mutagenesis: Exploring Novel Genes and Pathways, Book edited by: Tomlekova, N, Kozgar, I and Wani, R, ISBN: 9789086862344, Publisher: Wageningen Academic Publishers, The Netherlands, Publishing date: July 10, 2014
Mbambo, Z., Minnis-Ndimba, R., Pineda, C., Ndimba, B., Bado, S., Lin, J., ... Mehlo, L. (2014). Proton induced X-Ray emission and electron microscopy analysis of induced mutants of sorghum., Workflow;11179 Wageningen Academic Publishers. http://hdl.handle.net/10204/8418
Mbambo, Z, R Minnis-Ndimba, C Pineda, B Ndimba, S Bado, J Lin, Rachel K Chikwamba, and L Mehlo. "Proton induced X-ray emission and electron microscopy analysis of induced mutants of sorghum" In WORKFLOW;11179, n.p.: Wageningen Academic Publishers. 2014. http://hdl.handle.net/10204/8418.
Mbambo Z, Minnis-Ndimba R, Pineda C, Ndimba B, Bado S, Lin J, et al. Proton induced X-ray emission and electron microscopy analysis of induced mutants of sorghum.. Workflow;11179. [place unknown]: Wageningen Academic Publishers; 2014. [cited yyyy month dd]. http://hdl.handle.net/10204/8418.
Permission is granted to archive authors post print version of the book, Publisher: Wageningen Academic Publishers, The Netherlands, Publishing date: July 10, 2014
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