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dc.contributor.author Truter, JC
dc.contributor.author Van Wyk, JH
dc.contributor.author Oberholster, Paul J
dc.contributor.author Botha, AM
dc.date.accessioned 2014-07-18T10:03:38Z
dc.date.available 2014-07-18T10:03:38Z
dc.date.issued 2014-02
dc.identifier.citation Truter, J.C., Van Wyk, J.H., Oberholster, P.J. and Botha, A.M. 2014. The impacts of neutralized acid mine drainage contaminated water on the expression of selected endocrine-linked genes in juvenile Mozambique tilapia Oreochromis mossambicus exposed in vivo. Ecotoxicology and Environmental Safety, vol. 100, pp 209-217 en_US
dc.identifier.issn 0147-6513
dc.identifier.uri http://ac.els-cdn.com/S0147651313005009/1-s2.0-S0147651313005009-main.pdf?_tid=8ab65660-06ad-11e4-b145-00000aab0f6c&acdnat=1404830495_fed212ebfd2bea789ac8270633ad4e2e
dc.identifier.uri http://hdl.handle.net/10204/7515
dc.description Copyright: 2014 Elsevier. This is an ABSTRACT ONLY. The definitive version is published in Ecotoxicology and Environmental Safety,pp 209-217 en_US
dc.description.abstract Acid mine drainage (AMD) is a global environmental concern due to detrimental impacts on river ecosystems. Little is however known regarding the biological impacts of neutralized AMD on aquatic vertebrates despite excessive discharge into watercourses. The aim of this investigation was to evaluate the endocrine modulatory potential of neutralized AMD, using molecular biomarkers in the teleost fish Oreochromis mossambicus in exposure studies. Surface water was collected from six locations downstream of a high density sludge (HDS) AMD treatment plant and a reference site unimpacted by AMD. The concentrations of 28 elements, including 22 metals, were quantified in the exposure water in order to identify potential links to altered gene expression. Relatively high concentrations of manganese (~ 10mg/l), nickel (~ 0.1mg/l) and cobalt (~ 0.03 mg/l) were detected downstream of the HDS plant. The expression of thyroid receptor-a (tra), trß, androgen receptor-1 (ar1), ar2, glucocorticoid receptor-1 (gr1), gr2, mineralocorticoid receptor (mr) and aromatase (cyp19a1b) was quantified in juvenile fish after 48 h exposure. Slight but significant changes were observed in the expression of gr1 and mr in fish exposed to water collected directly downstream of the HDS plant, consisting of approximately 95 percent neutralized AMD. The most pronounced alterations in gene expression (i.e. tra, trß, gr1, gr2, ar1 and mr) was associated with water collected further downstream at a location with no other apparent contamination vectors apart from the neutralized AMD. The altered gene expression associated with the "downstream" locality coincided with higher concentrations of certain metals relative to the locality adjacent to the HDS plant which may indicate a causative link. The current study provides evidence of endocrine disruptive activity associated with neutralized AMD contamination in regard to alterations in the expression of key genes linked to the thyroid, interrenal and gonadal endocrine axes of a teleost fish species. en_US
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.relation.ispartofseries Workflow;12530
dc.subject Endocrine disruption en_US
dc.subject Gene expression en_US
dc.subject Metal ions en_US
dc.subject Acid mine drainage en_US
dc.title The impacts of neutralized acid mine drainage contaminated water on the expression of selected endocrine-linked genes in juvenile Mozambique tilapia Oreochromis mossambicus exposed in vivo en_US
dc.type Article en_US


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