dc.contributor.author |
Wilsenach, J
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dc.contributor.author |
Burke, L
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dc.contributor.author |
Radebe, V
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dc.contributor.author |
Mashego, M
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dc.contributor.author |
Stone, W
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dc.contributor.author |
Mouton, M
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dc.contributor.author |
Botha, A
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dc.date.accessioned |
2014-07-18T09:34:43Z |
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dc.date.available |
2014-07-18T09:34:43Z |
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dc.date.issued |
2014-01 |
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dc.identifier.citation |
Wilsenach, J, Burke, L, Radebe, V, Mashego, M, Stone, W, Mouton, M and Botha, A. 2014. Anaerobic ammonium oxidation in the old trickling filters at Daspooort wastewater treatment works. Water SA, vol. 40(1), pp 81-88 |
en_US |
dc.identifier.issn |
0378-4738 |
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dc.identifier.uri |
http://www.ajol.info/index.php/wsa/article/view/99709/88989
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dc.identifier.uri |
http://hdl.handle.net/10204/7495
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dc.description |
Copyright: 2014 Water Research Commission. This is an ABSTRACT ONLY. The definitive version is published in Water SA, vol. 40(1), pp 81-88 |
en_US |
dc.description.abstract |
The century-old trickling filters at the Daspoort Wastewater Treatment Works in Pretoria (Gauteng, South Africa) are known for their remarkable removal of nitrogen from municipal wastewater. Our study was conducted to identify the microbiological processes responsible for this phenomenon and to establish whether anammox bacteria may be involved. An aerobic and anaerobic bench top reactor, both inoculated with biofilm-covered stones from one of the filters, were spiked with ammonia-nitrogen (NH(sub4_(sup+)-N) and nitrite-nitrogen (NO(sub2)(sup-)-N). These reactors were subsequently monitored by conducting stoichiometric analyses of chemical oxygen demand (COD), NH(sub4)(sup+)-N, NO(sub2)(sup-)-N, and nitrate-nitrogen (NO(sub3)(sup-)-N). In the aerobic reactor, the COD concentration decreased over the 56 h batch reaction period and nitrification was revealed by a decrease in NH(sub4)(sup+)-N and NO(sub2)(sup-)-N concentrations. However, the NO(sub3)(sup-)-N concentration showed no notable decrease. In contrast, in the anaerobic reactor the concentrations of COD, NH(sub4)(sup+)-N, NO(sub2)¯-N, NO(sub3)¯-N, as well as total nitrogen decreased during the batch reaction period. The decrease of both the NH(sub4)(sup+)-N and NO(sub2)(sup-)-N concentrations, the latter to zero under anaerobic conditions, suggested that, in addition to heterotrophic denitrification, anaerobic ammonium oxidation (anammox) may also occur in the trickling filter biofilm. This was highlighted by the observation that ammonium removal in the anaerobic reactor stopped as soon as the nitrite concentration became zero. The ratio of nitrite:ammonium removal was 1.33 on average, which conforms to anammox behaviour. Gene sequence analysis was used to test for the possible presence of anammox bacteria in the trickling filter biofilm. Genomic DNA was extracted from trickling filter humus sludge and the polymerase chain reaction (PCR) was used to amplify taxonomically informative 16S rRNA gene sequences, using primers specific for selected anammox species. Subsequent sequence analysis, including using the online Basic Local Alignment Search Tool (BLAST), and constructing a phylogenic tree using a heuristic search strategy for Maximum Parsimony analysis, confirmed the presence of an anammox bacterium closely related to Candidatus ‘Brocadia anammoxidans’ and Candidatus ‘Brocadia fulgida’ on the biofilm-covered stones of the Daspoort trickling filters. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Water Research Commission |
en_US |
dc.relation.ispartofseries |
Workflow;12783 |
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dc.subject |
Daspoort Wastewater Treatment Works |
en_US |
dc.subject |
Trickling filter |
en_US |
dc.subject |
Anammox |
en_US |
dc.subject |
Nitrification |
en_US |
dc.subject |
Denitrification |
en_US |
dc.subject |
Stoichiometric analyses |
en_US |
dc.subject |
Polymerase chain reaction |
en_US |
dc.title |
Anaerobic ammonium oxidation in the old trickling filters at Daspooort wastewater treatment works |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
Wilsenach, J., Burke, L., Radebe, V., Mashego, M., Stone, W., Mouton, M., & Botha, A. (2014). Anaerobic ammonium oxidation in the old trickling filters at Daspooort wastewater treatment works. http://hdl.handle.net/10204/7495 |
en_ZA |
dc.identifier.chicagocitation |
Wilsenach, J, L Burke, V Radebe, M Mashego, W Stone, M Mouton, and A Botha "Anaerobic ammonium oxidation in the old trickling filters at Daspooort wastewater treatment works." (2014) http://hdl.handle.net/10204/7495 |
en_ZA |
dc.identifier.vancouvercitation |
Wilsenach J, Burke L, Radebe V, Mashego M, Stone W, Mouton M, et al. Anaerobic ammonium oxidation in the old trickling filters at Daspooort wastewater treatment works. 2014; http://hdl.handle.net/10204/7495. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Wilsenach, J
AU - Burke, L
AU - Radebe, V
AU - Mashego, M
AU - Stone, W
AU - Mouton, M
AU - Botha, A
AB - The century-old trickling filters at the Daspoort Wastewater Treatment Works in Pretoria (Gauteng, South Africa) are known for their remarkable removal of nitrogen from municipal wastewater. Our study was conducted to identify the microbiological processes responsible for this phenomenon and to establish whether anammox bacteria may be involved. An aerobic and anaerobic bench top reactor, both inoculated with biofilm-covered stones from one of the filters, were spiked with ammonia-nitrogen (NH(sub4_(sup+)-N) and nitrite-nitrogen (NO(sub2)(sup-)-N). These reactors were subsequently monitored by conducting stoichiometric analyses of chemical oxygen demand (COD), NH(sub4)(sup+)-N, NO(sub2)(sup-)-N, and nitrate-nitrogen (NO(sub3)(sup-)-N). In the aerobic reactor, the COD concentration decreased over the 56 h batch reaction period and nitrification was revealed by a decrease in NH(sub4)(sup+)-N and NO(sub2)(sup-)-N concentrations. However, the NO(sub3)(sup-)-N concentration showed no notable decrease. In contrast, in the anaerobic reactor the concentrations of COD, NH(sub4)(sup+)-N, NO(sub2)¯-N, NO(sub3)¯-N, as well as total nitrogen decreased during the batch reaction period. The decrease of both the NH(sub4)(sup+)-N and NO(sub2)(sup-)-N concentrations, the latter to zero under anaerobic conditions, suggested that, in addition to heterotrophic denitrification, anaerobic ammonium oxidation (anammox) may also occur in the trickling filter biofilm. This was highlighted by the observation that ammonium removal in the anaerobic reactor stopped as soon as the nitrite concentration became zero. The ratio of nitrite:ammonium removal was 1.33 on average, which conforms to anammox behaviour. Gene sequence analysis was used to test for the possible presence of anammox bacteria in the trickling filter biofilm. Genomic DNA was extracted from trickling filter humus sludge and the polymerase chain reaction (PCR) was used to amplify taxonomically informative 16S rRNA gene sequences, using primers specific for selected anammox species. Subsequent sequence analysis, including using the online Basic Local Alignment Search Tool (BLAST), and constructing a phylogenic tree using a heuristic search strategy for Maximum Parsimony analysis, confirmed the presence of an anammox bacterium closely related to Candidatus ‘Brocadia anammoxidans’ and Candidatus ‘Brocadia fulgida’ on the biofilm-covered stones of the Daspoort trickling filters.
DA - 2014-01
DB - ResearchSpace
DP - CSIR
KW - Daspoort Wastewater Treatment Works
KW - Trickling filter
KW - Anammox
KW - Nitrification
KW - Denitrification
KW - Stoichiometric analyses
KW - Polymerase chain reaction
LK - https://researchspace.csir.co.za
PY - 2014
SM - 0378-4738
T1 - Anaerobic ammonium oxidation in the old trickling filters at Daspooort wastewater treatment works
TI - Anaerobic ammonium oxidation in the old trickling filters at Daspooort wastewater treatment works
UR - http://hdl.handle.net/10204/7495
ER -
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en_ZA |