dc.contributor.author |
Adewusi, EA
|
|
dc.contributor.author |
Steenkamp, P
|
|
dc.contributor.author |
Fouché, Gerda
|
|
dc.contributor.author |
Steenkamp, V
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|
dc.date.accessioned |
2013-10-23T12:02:17Z |
|
dc.date.available |
2013-10-23T12:02:17Z |
|
dc.date.issued |
2013-06 |
|
dc.identifier.citation |
Adewusia, E.A, Steenkamp, P, Fouche, G and Steenkamp, V. 2013. Isolation of cycloeucalenol from Boophone disticha and evaluation of its cytotoxicity. Natural Product Communications, vol. 8(9), pp 1213-1216 |
en_US |
dc.identifier.issn |
1934-578X |
|
dc.identifier.issn |
1555-9475 |
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dc.identifier.uri |
http://hdl.handle.net/10204/6992
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|
dc.description |
Copyright: 2013 Natural Product Communications, vol. 8(9), pp 1213-1216 |
en_US |
dc.description.abstract |
Boophone disticha (Amaryllidaceae) is widely used in traditional medicine in southern Africa. Several alkaloids, volatile oils and fatty acids have been isolated from the plant. However, there has been no literature report of a triterpene from B. disticha. Cycloeucalenol, a cycloartane triterpene, together with its regioisomer, was isolated from the ethyl acetate extract of the bulbs using column chromatography and preparative thin layer chromatography. Structural elucidation was carried out using 1D and 2D NMR and mass spectroscopy. The MTT and neutral red assays were used to assess the cytotoxicity of the compound in human neuroblastoma (SH-SY5Y) cells. The compound was obtained as a mixture of two regio-isomers, which were separated for the first time by chromatographic optimization. Integration of the (sup1)H NMR spectrum showed that cycloeucalenol and its regio-isomer were present in a ratio of 1.04:1. A dose-dependent decrease in cell viability was observed using both cytotoxicity assays. IC(sub50) values of 173.0 ± 5.1 µM and 223.0 ± 6.4 µM were obtained for the MTT and neutral red assays, respectively, indicative of the low toxicity of the compound. This work describes for the first time, the presence of triterpene compounds from the genus Boophone. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Natural Product Communications |
en_US |
dc.relation.ispartofseries |
Workflow;11650 |
|
dc.subject |
Amaryllidaceae |
en_US |
dc.subject |
Boophone disticha |
en_US |
dc.subject |
Cycloeucalenol |
en_US |
dc.subject |
Cytotoxicity |
en_US |
dc.subject |
SH-SY5Y cells |
en_US |
dc.subject |
Regio-isomer |
en_US |
dc.subject |
Traditional plants |
en_US |
dc.title |
Isolation of cycloeucalenol from Boophone disticha and evaluation of its cytotoxicity |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
Adewusi, E., Steenkamp, P., Fouché, G., & Steenkamp, V. (2013). Isolation of cycloeucalenol from Boophone disticha and evaluation of its cytotoxicity. http://hdl.handle.net/10204/6992 |
en_ZA |
dc.identifier.chicagocitation |
Adewusi, EA, P Steenkamp, Gerda Fouché, and V Steenkamp "Isolation of cycloeucalenol from Boophone disticha and evaluation of its cytotoxicity." (2013) http://hdl.handle.net/10204/6992 |
en_ZA |
dc.identifier.vancouvercitation |
Adewusi E, Steenkamp P, Fouché G, Steenkamp V. Isolation of cycloeucalenol from Boophone disticha and evaluation of its cytotoxicity. 2013; http://hdl.handle.net/10204/6992. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Adewusi, EA
AU - Steenkamp, P
AU - Fouché, Gerda
AU - Steenkamp, V
AB - Boophone disticha (Amaryllidaceae) is widely used in traditional medicine in southern Africa. Several alkaloids, volatile oils and fatty acids have been isolated from the plant. However, there has been no literature report of a triterpene from B. disticha. Cycloeucalenol, a cycloartane triterpene, together with its regioisomer, was isolated from the ethyl acetate extract of the bulbs using column chromatography and preparative thin layer chromatography. Structural elucidation was carried out using 1D and 2D NMR and mass spectroscopy. The MTT and neutral red assays were used to assess the cytotoxicity of the compound in human neuroblastoma (SH-SY5Y) cells. The compound was obtained as a mixture of two regio-isomers, which were separated for the first time by chromatographic optimization. Integration of the (sup1)H NMR spectrum showed that cycloeucalenol and its regio-isomer were present in a ratio of 1.04:1. A dose-dependent decrease in cell viability was observed using both cytotoxicity assays. IC(sub50) values of 173.0 ± 5.1 µM and 223.0 ± 6.4 µM were obtained for the MTT and neutral red assays, respectively, indicative of the low toxicity of the compound. This work describes for the first time, the presence of triterpene compounds from the genus Boophone.
DA - 2013-06
DB - ResearchSpace
DP - CSIR
KW - Amaryllidaceae
KW - Boophone disticha
KW - Cycloeucalenol
KW - Cytotoxicity
KW - SH-SY5Y cells
KW - Regio-isomer
KW - Traditional plants
LK - https://researchspace.csir.co.za
PY - 2013
SM - 1934-578X
SM - 1555-9475
T1 - Isolation of cycloeucalenol from Boophone disticha and evaluation of its cytotoxicity
TI - Isolation of cycloeucalenol from Boophone disticha and evaluation of its cytotoxicity
UR - http://hdl.handle.net/10204/6992
ER -
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en_ZA |