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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/6325

Title: Selection and application of ssDNA aptamers to detect active TB from sputum samples
Authors: Rotherham, LS
Maserumule, C
Dheda, K
Theron, J
Khati, M
Keywords: Tuberculosis
Sputum samples
Tuberculosis diagnosis
Issue Date: Oct-2012
Publisher: Public Library of Science
Citation: Rotherham, LS, Maserumule, C, Dheda, K, Theron, J and Khathi, M. Selection and application of ssDNA aptamers to detect active TB from sputum samples. Plos One, vol. 7(10), pp 1-11
Series/Report no.: Workflow;9684
Abstract: Despite the enormous global burden of tuberculosis (TB), conventional approaches to diagnosis continue to rely on tests that have major drawbacks. The improvement of TB diagnostics relies, not only on good biomarkers, but also upon accurate detection methodologies. The 10-kDa culture filtrate protein (CFP-10) and the 6-kDa early secreted antigen target (ESAT-6) are potent T-cell antigens that are recognised by over 70% of TB patients. Aptamers, a novel sensitive and specific class of detection molecules, has hitherto, not been raised to these relatively TB-specific antigens. This preliminary proof-of-concept study suggests that a diagnosis of active TB using anti-CFP-10.ESAT-6 aptamers applied to human sputum samples is feasible.
Description: Copyright: 2012 Rotherham et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
URI: http://www.plosone.org/article/related/info%3Adoi%2F10.1371%2Fjournal.pone.0046862;jsessionid=09BE946219E7313CA3F1965F6455C593
ISSN: 1932-6203
Appears in Collections:Accessibility research
General science, engineering & technology
Aptamer technology

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