DSpace
 

Researchspace >
General science, engineering & technology >
General science, engineering & technology >
General science, engineering & technology >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/5970

Title: A feruloyl esterase derived from a leachate metagenome library
Authors: Rashamuse, K
Sanyika, W
Ronneburg, T
Brady, D
Keywords: Feruloyl esterase
Lipolytic enzymes
Metagenomics
Issue Date: Jan-2012
Publisher: Korean Society for Biochemistry and Molecular Biology
Citation: Rashamuse, K, Sanyika, W, Ronneburg, T and Brady, D. 2012. A feruloyl esterase derived from a leachate metagenome library. BMB Reports, vol. 45(1), pp 14-19
Series/Report no.: Workflow;7184
Abstract: A feruloyl esterase encoding gene (designated fae6), derived from a leachate metagenomic library, was cloned and the nucleotide sequence of the insert DNA determined. Translational analysis revealed that fae6 consists of a 515 amino acid polypeptide, encoding a 55 kDa pre-protein. The Fae6 primary structure contained the G-E-S-A-G sequence, which corresponds well with a typical catalytic serine sequence motif (G-x-S-x-G). The fae6 gene was successfully over-expressed in E. coli and the recombinant protein was purified to 8.4 fold enrichment with 17% recovery. The KM data showed Fae6 has a high affinity to methyl sinapate while thermostability data indicated that Fae6 was thermolabile with a half life (T1/2) < 30 min at 50oC. High affinity for Fae6 against methyl sinapate, methyl ferulate and ethyl ferulate suggest that the enzyme can be useful in hydrolyzing ferulated polysaccharides in a biorefinery process.
Description: Copyright: 2012 Korean Society for Biochemistry and Molecular Biology
URI: http://bmbreports.org/jbmb/jbmb_files/%5B45-1%5D1201272127_(014-019)BMB11-165.pdf
http://hdl.handle.net/10204/5970
ISSN: 1976-6696
Appears in Collections:Pollution and waste
General science, engineering & technology

Files in This Item:

File Description SizeFormat
Rashamuse1_2012.pdf875.53 kBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback