dc.contributor.author |
Van Zyl, WH
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|
dc.contributor.author |
Lodolo, EJ
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|
dc.contributor.author |
Gericke, M
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|
dc.date.accessioned |
2007-06-12T07:48:48Z |
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dc.date.available |
2007-06-12T07:48:48Z |
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dc.date.issued |
1993-04 |
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dc.identifier.citation |
Van Zyl, WH, Lodolo, EJ and Gericke, M. 1993. Conversion of homothallic yeast to heterothallism trough HO gene disruption. Current Genetics, vol. 23(4), pp 290-294 |
en |
dc.identifier.issn |
0172-8083 |
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dc.identifier.uri |
http://hdl.handle.net/10204/582
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dc.description |
Copyright: 1993 Springer-Verlag |
en |
dc.description.abstract |
A simple method was developed for the conversion of homothallic Saccharomyces cerevisiae yeaststrains to heterothallism through HO gene disruption. An integrative ho:: neo disrupted allele was constructed by cloning a dominant selectable marker, the bacterial neo gene, within the HO gene. Transformation of a homothallic diploid S. cerevisiae strain with plasmid DNA containing the ho:: neo allele yielded G418-resistantyeast transformants in which one of the HO alleles was replaced by the disrupted ho: : neo allele. Meiotic tetrad analysis of four-spored asci from these G418-resistanttransformants gave rise to haploid heterothallic and diploid homothallic tetrad progeny. The presence of the ho:: neo and HO alleles in the heterothallic and homothallic progeny was confirmed by Southern-blot analysis. |
en |
dc.language.iso |
en |
en |
dc.publisher |
Springer-Verlag |
en |
dc.subject |
G418 resistance |
en |
dc.subject |
S. cerevisiae |
en |
dc.subject |
Homothallism |
en |
dc.subject |
HO gene disruptions |
en |
dc.title |
Conversion of homothallic yeast to heterothallism trough HO gene disruption |
en |
dc.type |
Article |
en |
dc.identifier.apacitation |
Van Zyl, W., Lodolo, E., & Gericke, M. (1993). Conversion of homothallic yeast to heterothallism trough HO gene disruption. http://hdl.handle.net/10204/582 |
en_ZA |
dc.identifier.chicagocitation |
Van Zyl, WH, EJ Lodolo, and M Gericke "Conversion of homothallic yeast to heterothallism trough HO gene disruption." (1993) http://hdl.handle.net/10204/582 |
en_ZA |
dc.identifier.vancouvercitation |
Van Zyl W, Lodolo E, Gericke M. Conversion of homothallic yeast to heterothallism trough HO gene disruption. 1993; http://hdl.handle.net/10204/582. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Van Zyl, WH
AU - Lodolo, EJ
AU - Gericke, M
AB - A simple method was developed for the conversion of homothallic Saccharomyces cerevisiae yeaststrains to heterothallism through HO gene disruption. An integrative ho:: neo disrupted allele was constructed by cloning a dominant selectable marker, the bacterial neo gene, within the HO gene. Transformation of a homothallic diploid S. cerevisiae strain with plasmid DNA containing the ho:: neo allele yielded G418-resistantyeast transformants in which one of the HO alleles was replaced by the disrupted ho: : neo allele. Meiotic tetrad analysis of four-spored asci from these G418-resistanttransformants gave rise to haploid heterothallic and diploid homothallic tetrad progeny. The presence of the ho:: neo and HO alleles in the heterothallic and homothallic progeny was confirmed by Southern-blot analysis.
DA - 1993-04
DB - ResearchSpace
DP - CSIR
KW - G418 resistance
KW - S. cerevisiae
KW - Homothallism
KW - HO gene disruptions
LK - https://researchspace.csir.co.za
PY - 1993
SM - 0172-8083
T1 - Conversion of homothallic yeast to heterothallism trough HO gene disruption
TI - Conversion of homothallic yeast to heterothallism trough HO gene disruption
UR - http://hdl.handle.net/10204/582
ER -
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en_ZA |