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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/5647

Title: Investigation of in vitro and in vivo anti-asthmatic properties of Siphonochilus aethiopicus
Authors: Fouche, G
Nieuwenhuizen, N
Maharaj, V
Van Rooyen, S
Harding, N
Nthambeleni, R
Jayakumar, J
Kirstein, F
Emedi, B
Meoni, P
Keywords: Asthma
Siphonochilus aethiopicus
Medicinal plants
Issue Date: Jan-2011
Publisher: Elsevier
Citation: Fouche, G, Nieuwenhuizen, N, Maharaj, V, Van Rooyen, S, Harding, N, Nthambeleni, R, Jayakumar, J, Kirstein, F, Emedi, B and Meoni, P. 2011. Investigation of in vitro and in vivo anti-asthmatic properties of Siphonochilus aethiopicus. Journal of Ethnopharmacology, vol. 133(2), pp 843-849
Series/Report no.: Workflow;4310
Abstract: Asthma is a chronic inflammatory disease of the lungs, characterized by increased sensitivity to bronchoconstriction associated with infiltration of immune cells, mucus hypersecretion and structural remodelling of the airways. In South Africa, the indigenous plant Siphonochilus aethiopicus, is used by traditional health practitioners to treat colds, wheezing of the chest, coughs, influenza, sinus problems and mild asthma. In this study we aimed to investigate the potential anti-inflammatory and anti-allergic properties of S. aethiopicus in vitro and its efficacy in a mouse model of allergic asthma. Materials and methods: The dried and powdered S. aethiopicus plant material was extracted separately with organic solvents (diethyl ether, ethanol) and water. Dried extracts as well as a purified furanoterpenoid compound present in the extracts were screened in vitro in a glucocorticoid and histamine H1 receptor binding assay and a phosphodiesterase IV enzyme inhibition assay. Extracts were also evaluated for efficacy against ovalbumin (OVA)-induced allergic airway disease in mice. Results: Biological assaying of extracts of the plant and the isolated furanoterpenoid showed significant in vitro inhibition of glucocorticoid and histamine H1 receptor binding and phosphodiesterase IV activity, supporting a possible anti-inflammatory, anti-allergic and bronchodilatory effects. Administration of S. aethiopicus extracts to OVA-sensitized and challenged mice significantly reduced lung inflammation and the percentage of eosinophils in bronchoalveolar lavage fluid but did not influence airway hyper reactivity. Conclusion:this study provides evidence that S. aethiopicus has anti-inflammatory and anti-allergic properties in vitro and in vivo. These findings may support anecdotal accounts of its effectiveness against asthma, sinusitis, colds and flu.
Description: Copyright: 2011 Elsevier. This is the pre-print version of the work. The definitive version is published in Journal of Ethnopharmacology, vol. 133(2), pp 843-849
URI: http://www.sciencedirect.com/science/article/pii/S0378874110007993
ISSN: 0378-8741
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General science, engineering & technology

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