dc.contributor.author |
Oberholster, Paul J
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dc.contributor.author |
Botha, AM
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dc.date.accessioned |
2011-07-12T12:21:44Z |
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dc.date.available |
2011-07-12T12:21:44Z |
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dc.date.issued |
2010-12 |
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dc.identifier.citation |
Oberholster, P.J., and Botha, A.M. 2010. Use of remote sensing and molecular markers to detect toxic cyanobacterial hyperscum crust: A case study on Lake Hartbeespoort, South Africa. African Journal of Biotechnology, Vol. 9(51), pp 8791-8799 |
en_US |
dc.identifier.issn |
1684-5315 |
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dc.identifier.uri |
http://www.academicjournals.org/AJB/abstracts/abs2010/20Dec/Oberholster%20and%20Botha.htm
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dc.identifier.uri |
http://hdl.handle.net/10204/5094
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dc.description |
Copyright: 2010 Academic Journals. This is a post print version of the work. The definitive version is published in the African Journal of Biotechnology, Vol. 9(51), pp 8791-8799 |
en_US |
dc.description.abstract |
In this study, we monitored the formation of cyanobacterial hyperscum and crust formation in Lake Hartbeespoort using satellite images and ground monitoring. The hyperscum that formed near the reservoir wall was characterised by a distinctive white surface layer of crust. Hyperscum is the result of exposure of the cells to high radiation, inflicting irreversible damage to the genetic constitution of the upper layer of Microcystis aeruginosa cells. Under the 3 mm thick layer of crust, dark (<0.93 μmol of photons m-2s-1) anaerobic conditions (0.4 mg/l, 3% saturation) prevailed with high levels of microcystin (12,300 μg/l) in the absence of sunlight irradiation and photolysis by UV light. Real time polymerase chain reaction (PCR) analysis indicated low levels of transcription of the mcyA, mcyB and mcyD genes which are responsible for synthesis of cyanotoxins under these low light intensity conditions. At other sampling sites where cyanobacterial scum occurred and hyperscum crust was absent, only the mcyB and mcyD genes were transcribed. A plausible explanation for the transcription of the mcyA gene in the hyperscum and not at the other sampling sites, was the presence of environmental stress-inducing factors, e.g. low light intensity (0.93 μmol of photon m-2 s-1) and pH 6.1. At the sampling site where no cyanobacterial scum was visible on the satellite images, low cell abundance (2.4 x 104 μg/l) and chlorophyll a (12.2 μg/l) was measured in comparison with sites where cyanobacterial scum was visible on the satellite images. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Academic Journals |
en_US |
dc.relation.ispartofseries |
Workflow;5489 |
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dc.subject |
Hyperscum crust |
en_US |
dc.subject |
Reverse-transcription PCR |
en_US |
dc.subject |
McyA levels |
en_US |
dc.subject |
Microcystin |
en_US |
dc.subject |
Satellite imaging |
en_US |
dc.subject |
Cyanobacteria |
en_US |
dc.title |
Use of remote sensing and molecular markers to detect toxic cyanobacterial hyperscum crust: A case study on Lake Hartbeespoort, South Africa |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
Oberholster, P. J., & Botha, A. (2010). Use of remote sensing and molecular markers to detect toxic cyanobacterial hyperscum crust: A case study on Lake Hartbeespoort, South Africa. http://hdl.handle.net/10204/5094 |
en_ZA |
dc.identifier.chicagocitation |
Oberholster, Paul J, and AM Botha "Use of remote sensing and molecular markers to detect toxic cyanobacterial hyperscum crust: A case study on Lake Hartbeespoort, South Africa." (2010) http://hdl.handle.net/10204/5094 |
en_ZA |
dc.identifier.vancouvercitation |
Oberholster PJ, Botha A. Use of remote sensing and molecular markers to detect toxic cyanobacterial hyperscum crust: A case study on Lake Hartbeespoort, South Africa. 2010; http://hdl.handle.net/10204/5094. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Oberholster, Paul J
AU - Botha, AM
AB - In this study, we monitored the formation of cyanobacterial hyperscum and crust formation in Lake Hartbeespoort using satellite images and ground monitoring. The hyperscum that formed near the reservoir wall was characterised by a distinctive white surface layer of crust. Hyperscum is the result of exposure of the cells to high radiation, inflicting irreversible damage to the genetic constitution of the upper layer of Microcystis aeruginosa cells. Under the 3 mm thick layer of crust, dark (<0.93 μmol of photons m-2s-1) anaerobic conditions (0.4 mg/l, 3% saturation) prevailed with high levels of microcystin (12,300 μg/l) in the absence of sunlight irradiation and photolysis by UV light. Real time polymerase chain reaction (PCR) analysis indicated low levels of transcription of the mcyA, mcyB and mcyD genes which are responsible for synthesis of cyanotoxins under these low light intensity conditions. At other sampling sites where cyanobacterial scum occurred and hyperscum crust was absent, only the mcyB and mcyD genes were transcribed. A plausible explanation for the transcription of the mcyA gene in the hyperscum and not at the other sampling sites, was the presence of environmental stress-inducing factors, e.g. low light intensity (0.93 μmol of photon m-2 s-1) and pH 6.1. At the sampling site where no cyanobacterial scum was visible on the satellite images, low cell abundance (2.4 x 104 μg/l) and chlorophyll a (12.2 μg/l) was measured in comparison with sites where cyanobacterial scum was visible on the satellite images.
DA - 2010-12
DB - ResearchSpace
DP - CSIR
KW - Hyperscum crust
KW - Reverse-transcription PCR
KW - McyA levels
KW - Microcystin
KW - Satellite imaging
KW - Cyanobacteria
LK - https://researchspace.csir.co.za
PY - 2010
SM - 1684-5315
T1 - Use of remote sensing and molecular markers to detect toxic cyanobacterial hyperscum crust: A case study on Lake Hartbeespoort, South Africa
TI - Use of remote sensing and molecular markers to detect toxic cyanobacterial hyperscum crust: A case study on Lake Hartbeespoort, South Africa
UR - http://hdl.handle.net/10204/5094
ER -
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en_ZA |