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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/4940

Title: Electron transfer dynamics across self-assembled N-(2-mercaptoethyl) octadecanamide/mycolic acid layers: impedimetric insights into the structural integrity and interaction with anti-mycolic acid antibodies
Authors: Ozoemena, KI
Mathebula, NS
Pillay, J
Toschi, G
Verschoor, JA
Keywords: Au–MEODA
Mycolic acid
Gold electrode
Au–MEODA–MA
TB detection
MA-anti-MA
Electron transfer
Impedance spectroscopy
Voltammetry
Chemistry chemical physics
Chemistry chemical
Issue Date: 2010
Publisher: The Royal Society of Chemistry
Citation: Ozoemena, KI, Mathebula, NS, Pillay, J. 2010. Electron transfer dynamics across self-assembled N-(2-mercaptoethyl) octadecanamide/mycolic acid layers: impedimetric insights into the structural integrity and interaction with anti-mycolic acid antibodies. Physical Chemistry Chemical Physics, Vol. 12(2), pp 345-357
Series/Report no.: Workflow request;6123
Abstract: The integrity and properties of mycolic acid (MA) antigens integrated into a self-assembled monolayer (SAM) of N-(2-mercaptoethyl)octadecanamide, (MEODA), on a gold electrode have been interrogated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). EIS data showed that Au–MEODA and Au–MEODA–MA behave as microelectrode arrays, with pinholes acting as the microelectrodes that permit electron transport between a redox-active probe in solution and the underlying gold surface. The average radii of the pinholes (ra) and half the distance between the centers of the neighbouring pinholes (rb), were estimated from EIS using the pore size model, and discussed. Anti-MA antibodies present in a tuberculosis (TB)-infected patient (co-infected with HIV) strongly interact with Au–MEODA–MA showing a rather compact and stable bio-complex structure that is virtually defect-free. The electrochemical impedimetric properties associated with the ability of the Au–MEODA–MA to discriminate between TB positive and negative human sera are also discussed. We prove that the Au–MEODA and Au–MEODA–MA electrodes, as well as the MA-anti-MA antibody interactions, are characterized with time-constant dispersion, typical of microstructures with grain/grain boundary phases. These crucial physico-electrochemical insights into the behaviour of surface-confined MA should provide a useful basis for the design and development of a potential impedimetric immunosensing platform for active tuberculosis.
Description: Copyright: 2010 The Royal Society of Chemistry
URI: http://hdl.handle.net/10204/4940
Appears in Collections:Nanotechnology
General science, engineering & technology

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