dc.contributor.author |
Rolando, M
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dc.contributor.author |
Stefani, C
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dc.contributor.author |
Flatau, G
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dc.contributor.author |
Auberger, P
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dc.contributor.author |
Mettouchi, A
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dc.contributor.author |
Mhlanga, Musa
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dc.contributor.author |
Rapp, U
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dc.contributor.author |
Galmiche, A
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dc.contributor.author |
Lemichez, E
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dc.date.accessioned |
2010-11-11T11:00:15Z |
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dc.date.available |
2010-11-11T11:00:15Z |
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dc.date.issued |
2010-07 |
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dc.identifier.citation |
Rolando, M, Stefani, C, Flatau, G et al. 2010. Transcriptome dysregulation by anthrax lethal toxin plays a key role in induction of human endothelial cell cytotoxicity. Cellular Microbiology, Vol. 12(7), pp 891-905 |
en |
dc.identifier.issn |
1462-5814 |
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dc.identifier.uri |
http://onlinelibrary.wiley.com/doi/10.1111/j.1462-5822.2010.01438.x/pdf
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dc.identifier.uri |
http://hdl.handle.net/10204/4551
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dc.description |
Copyright: 2010 Wiley Blackwell. This is the post print version of the work. The definitive version is published in Cellular Microbiology, Vol. 12(7), pp 891-905 |
en |
dc.description.abstract |
The authors have investigated how Bacillus anthracis lethal toxin (LT) triggers caspase-3 activation and the formation of thick actin cables in human endothelial cells. By DNA array analysis they show that LT has a major impact on the cell transcriptome and they identify key host genes involved in LT cytotoxic effects. Indeed, upregulation of TRAIL and downregulation of XIAP both participate in LT-induced caspase-3 activation. LT induces a downregulation of the immediate early gene and master regulator of transcription egr1. Importantly, its re-expression in LT-intoxicated cells blocks caspase-3 activation. In parallel, they found that the formation of actin cables induced by LT occurs in the absence of direct activation of RhoA/ROCK signalling. They show that knock-down of cortactin and rhophilin-2 under conditions of calponin-1 expression defines the minimal set of genes regulated by LT for actin cable formation. Together their data establish that the modulation of the cell transcriptome by LT plays a key role in triggering human endothelial cell toxicity. |
en |
dc.language.iso |
en |
en |
dc.publisher |
Wiley-Blackwell |
en |
dc.relation.ispartofseries |
Journal Article |
en |
dc.subject |
Lethal toxin |
en |
dc.subject |
Anthrax |
en |
dc.subject |
Human endothelial cell |
en |
dc.subject |
Cell toxicity |
en |
dc.subject |
Bacillus anthracis |
en |
dc.subject |
Cellular microbiology |
en |
dc.title |
Transcriptome dysregulation by anthrax lethal toxin plays a key role in induction of human endothelial cell cytotoxicity |
en |
dc.type |
Article |
en |
dc.identifier.apacitation |
Rolando, M., Stefani, C., Flatau, G., Auberger, P., Mettouchi, A., Mhlanga, M., ... Lemichez, E. (2010). Transcriptome dysregulation by anthrax lethal toxin plays a key role in induction of human endothelial cell cytotoxicity. http://hdl.handle.net/10204/4551 |
en_ZA |
dc.identifier.chicagocitation |
Rolando, M, C Stefani, G Flatau, P Auberger, A Mettouchi, Musa Mhlanga, U Rapp, A Galmiche, and E Lemichez "Transcriptome dysregulation by anthrax lethal toxin plays a key role in induction of human endothelial cell cytotoxicity." (2010) http://hdl.handle.net/10204/4551 |
en_ZA |
dc.identifier.vancouvercitation |
Rolando M, Stefani C, Flatau G, Auberger P, Mettouchi A, Mhlanga M, et al. Transcriptome dysregulation by anthrax lethal toxin plays a key role in induction of human endothelial cell cytotoxicity. 2010; http://hdl.handle.net/10204/4551. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Rolando, M
AU - Stefani, C
AU - Flatau, G
AU - Auberger, P
AU - Mettouchi, A
AU - Mhlanga, Musa
AU - Rapp, U
AU - Galmiche, A
AU - Lemichez, E
AB - The authors have investigated how Bacillus anthracis lethal toxin (LT) triggers caspase-3 activation and the formation of thick actin cables in human endothelial cells. By DNA array analysis they show that LT has a major impact on the cell transcriptome and they identify key host genes involved in LT cytotoxic effects. Indeed, upregulation of TRAIL and downregulation of XIAP both participate in LT-induced caspase-3 activation. LT induces a downregulation of the immediate early gene and master regulator of transcription egr1. Importantly, its re-expression in LT-intoxicated cells blocks caspase-3 activation. In parallel, they found that the formation of actin cables induced by LT occurs in the absence of direct activation of RhoA/ROCK signalling. They show that knock-down of cortactin and rhophilin-2 under conditions of calponin-1 expression defines the minimal set of genes regulated by LT for actin cable formation. Together their data establish that the modulation of the cell transcriptome by LT plays a key role in triggering human endothelial cell toxicity.
DA - 2010-07
DB - ResearchSpace
DP - CSIR
KW - Lethal toxin
KW - Anthrax
KW - Human endothelial cell
KW - Cell toxicity
KW - Bacillus anthracis
KW - Cellular microbiology
LK - https://researchspace.csir.co.za
PY - 2010
SM - 1462-5814
T1 - Transcriptome dysregulation by anthrax lethal toxin plays a key role in induction of human endothelial cell cytotoxicity
TI - Transcriptome dysregulation by anthrax lethal toxin plays a key role in induction of human endothelial cell cytotoxicity
UR - http://hdl.handle.net/10204/4551
ER -
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en_ZA |