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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/4469

Title: Downstream process for production of a viable and stable Bacillus cereus aquaculture biological agent
Authors: Lalloo, R
Maharajh, D
Görgens, J
Gardiner, N
Keywords: Bacillus cereus
Downstream processing
Biological agent
Aquaculture
Issue Date: Mar-2010
Publisher: Springer
Citation: Lalloo, R, Maharajh, D, Görgens, J and Gardiner, N. 2010. Downstream process for production of a viable and stable Bacillus cereus aquaculture biological agent. Applied Microbiology and Biotechnology, Vol. 86(2), pp 499-508
Series/Report no.: Journal Article
Abstract: Biological products offer advantages over chemotherapeutics in aquaculture. Adoption in commercial application is lacking due to limitations in process and product development that address key end user product requirements such as cost, efficacy, shelf life and convenience. In previous studies, we have reported on the efficacy, physiological robustness and low-cost spore production of a Bacillus cereus isolate (NRRL 100132). This study examines the development of suitable spore recovery, drying, formulation and tablet production from the fermentation product. Key criteria used for such downstream process unit evaluation included spore viability, recovery, spore balance, spore re-germination, product intermediate stability, end product stability and efficacy. A process flow sheet comprising vertical tube centrifugation, fluidised bed agglomeration and tablet pressing yielded a suitable product. The formulation included corn steep liquor and glucose to enhance subsequent spore regermination. Viable spore recovery and spore balance closure across each of the process units was high (>70% and >99% respectively), with improvement in recovery possible by adoption of continuous processing at large scale. Spore regermination was 97%, whilst a product half-life in excess of 5 years was estimated based on thermal resistance curves. The process resulted in a commercially attractive product and suitable variable cost of production.
Description: Copyright: 2010 Springer. This is the author's pre print version of the work. The definitive version is published in Applied Microbiology and Biotechnology, Vol. 86(2), pp 499-508
URI: http://www.springerlink.com/content/353252416857p725/fulltext.pdf
http://hdl.handle.net/10204/4469
ISSN: 0175-7598
Appears in Collections:Manufacturing science and technology
General science, engineering & technology
Agroprocessing and chemical technology

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