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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/3689

Title: Femtosecond pump probe spectroscopy for the study of energy transfer of light-harvesting complexes from extractions of spinach leaves
Authors: Ombinda-Lemboumba, S
Du Plessis, A
Sparrow, RW
Molukanele, P
Botha, LR
Rohwer, EG
Steenkamp, CM
Van Rensburg, L
Keywords: Pump probe spectroscopy
Femtosecond laser
Ultrafast spectroscopy
Transient absorption
Light-harvesting complex II
Malachite green
Issue Date: Sep-2009
Publisher: Academy of Science of South Africa
Citation: Ombinda-Lemboumba, S, Du Plessis, A, Sparrow, RW et al. 2009. Femtosecond pump probe spectroscopy for the study of energy transfer of light-harvesting complexes from extractions of spinach leaves. South African Journal of Science, Vol. 105(9/10), pp 376-386
Abstract: Measurements of ultrafast transient processes, of temporal durations in the picosecond and femtosecond regime, are made possible by femtosecond pump probe transient absorption spectroscopy. Such an ultrafast pump probe transient absorption setup has been implemented at the CSIR National Laser Centre and has been applied to investigate energy transfer processes in different parts of photosynthetic systems. In this paper, researchers report on the first results obtained with Malachite green as a benchmark. Malachite green was chosen because the lifetime of its excited state is well known. Researchers also present experimental results of the ultrafast energy transfer of light-harvesting complexes in samples prepared from spinach leaves. Various pump wavelengths in the range 600–680 nm were used; the probe was a white light continuum spanning 420–700 nm. The experimental setup is described in detail in this paper. Results obtained with these samples are consistent with those expected and achieved by other researchers in this field.
Description: Copyright: 2009 Academy of Science of South Africa
URI: http://hdl.handle.net/10204/3689
ISSN: 0038-2353
Appears in Collections:Laser physics and technology
General science, engineering & technology
General research interest

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