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Please use this identifier to cite or link to this item: http://hdl.handle.net/10204/1514

Title: Correlation between the physicochemical properties of organic solvents and their biocompatibility toward epoxide hydrolase activity in whole-cells of a yeast, Rhodotorulasp
Authors: Lotter, J
Botes, AL
Van Dyk, MS
Breytenbach, JC
Keywords: Biotransformations
Co-solvents
Epoxide hydrolases
Yeasts
Issue Date: Aug-2004
Publisher: Kluwer Academic Publisher
Citation: Lotter, J, et al. 2004. Correlation between the physicochemical properties of organic solvents and their biocompatibility toward epoxide hydrolase activity in whole-cells of a yeast, Rhodotorulasp. Biotechnology Letters, vol 26 (15), pp 1191-1195
Abstract: Epoxides are often highly hydrophobic substrates and the presence of an organic co-solvent within an aqueous bioreactor is in such cases indicated. The effect of 40 water-miscible and -immiscible organic solvents on epoxide hydrolase activity in whole-cells of the yeast Rhodotorula sp. UOFS Y-0448 was investigated. No formal correlation between solvent biocompatibility and physicochemical properties was deductible, although the introduction of hydroxyl groups increased biocompatibility. 1-Pentanol, 2-methylcyclohexanol and 1-octanol were the most biocompatible resulting in relatively low activity losses when used at up to 20% (v/v).
URI: http://hdl.handle.net/10204/1514
http://hdl.handle.net/10204/1514
ISSN: 0141-5492
Appears in Collections:Yeast expression systems
General science, engineering & technology

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