dc.contributor.author |
Griffith, Derek J
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|
dc.contributor.author |
Bone, Emma L
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|
dc.contributor.author |
Thomalla, Sandy J
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|
dc.contributor.author |
Bernard, Stewart
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|
dc.date.accessioned |
2019-04-02T07:30:54Z |
|
dc.date.available |
2019-04-02T07:30:54Z |
|
dc.date.issued |
2018-07 |
|
dc.identifier.citation |
Griffith, D.J. et al. 2018. Calibration of an in-water multi-excitation fluorometer for the measurement of phytoplankton chlorophyll-a fluorescence quantum yield. Optics Express, vol. 26(15): 18863-18877 |
en_US |
dc.identifier.issn |
1094-4087 |
|
dc.identifier.uri |
https://www.osapublishing.org/oe/abstract.cfm?uri=oe-26-15-18863&origin=search
|
|
dc.identifier.uri |
https://doi.org/10.1364/OE.26.018863
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|
dc.identifier.uri |
http://hdl.handle.net/10204/10899
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|
dc.description |
This is an open access article published in Optics Express, vol. 26(15): 18863-18877 |
en_US |
dc.description.abstract |
A multi-excitation fluorometer (MFL, JFE Advantech Co., Ltd.), originally designed to discriminate between phytoplankton species present within a population, has been redirected for use in fluorescence quantum yield (FQY) determination. While this calibration for apparent FQY requires no modification of the MFL, it is necessary to have an independent measurement of the spectral absorption coefficient of the subject fluid. Two different approaches to calibration were implemented. The primary method made use of reference fluorescent dye solutions of known quantum yield. The second method made use of acrylic fluorescent plaques and films. The two methods yielded consistent results, except in the 570 and 590 nm LED channels of the MFL. Application of the MFL in FQY determination is illustrated with an in situ Southern Ocean sample. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
OSA Publishing |
en_US |
dc.relation.ispartofseries |
Worklist;22312 |
|
dc.relation.ispartofseries |
Worklist;22289 |
|
dc.subject |
Multi-excitation fluorometer |
en_US |
dc.subject |
MFL |
en_US |
dc.subject |
Phytoplankton species |
en_US |
dc.title |
Calibration of an in-water multi-excitation fluorometer for the measurement of phytoplankton chlorophyll-a fluorescence quantum yield |
en_US |
dc.type |
Article |
en_US |
dc.identifier.apacitation |
Griffith, D. J., Bone, E. L., Thomalla, S. J., & Bernard, S. (2018). Calibration of an in-water multi-excitation fluorometer for the measurement of phytoplankton chlorophyll-a fluorescence quantum yield. http://hdl.handle.net/10204/10899 |
en_ZA |
dc.identifier.chicagocitation |
Griffith, Derek J, Emma L Bone, Sandy J Thomalla, and Stewart Bernard "Calibration of an in-water multi-excitation fluorometer for the measurement of phytoplankton chlorophyll-a fluorescence quantum yield." (2018) http://hdl.handle.net/10204/10899 |
en_ZA |
dc.identifier.vancouvercitation |
Griffith DJ, Bone EL, Thomalla SJ, Bernard S. Calibration of an in-water multi-excitation fluorometer for the measurement of phytoplankton chlorophyll-a fluorescence quantum yield. 2018; http://hdl.handle.net/10204/10899. |
en_ZA |
dc.identifier.ris |
TY - Article
AU - Griffith, Derek J
AU - Bone, Emma L
AU - Thomalla, Sandy J
AU - Bernard, Stewart
AB - A multi-excitation fluorometer (MFL, JFE Advantech Co., Ltd.), originally designed to discriminate between phytoplankton species present within a population, has been redirected for use in fluorescence quantum yield (FQY) determination. While this calibration for apparent FQY requires no modification of the MFL, it is necessary to have an independent measurement of the spectral absorption coefficient of the subject fluid. Two different approaches to calibration were implemented. The primary method made use of reference fluorescent dye solutions of known quantum yield. The second method made use of acrylic fluorescent plaques and films. The two methods yielded consistent results, except in the 570 and 590 nm LED channels of the MFL. Application of the MFL in FQY determination is illustrated with an in situ Southern Ocean sample.
DA - 2018-07
DB - ResearchSpace
DP - CSIR
KW - Multi-excitation fluorometer
KW - MFL
KW - Phytoplankton species
LK - https://researchspace.csir.co.za
PY - 2018
SM - 1094-4087
T1 - Calibration of an in-water multi-excitation fluorometer for the measurement of phytoplankton chlorophyll-a fluorescence quantum yield
TI - Calibration of an in-water multi-excitation fluorometer for the measurement of phytoplankton chlorophyll-a fluorescence quantum yield
UR - http://hdl.handle.net/10204/10899
ER -
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en_ZA |