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Browsing by Author "Steenkamp, Lucia H"

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Alternative pathway implicated as an influencing factor in the synthesis of theaflavin

Author: Van der Westhuizen, M ; Steenkamp, Lucia H ; Steenkamp, P ; Apostolides, Z Date: Mar 2016 The principal pigments present in black tea, theaflavins (TF), have been indicated to be of potential clinical significance in various fields of research which has been hampered by the very low levels of TFs from black tea extractions, being ... Read more
Ambrafuran (AmbroxTM) synthesis from natural plant product precursors

Author: Ncube, EN ; Steenkamp, Lucia H ; Dubery, IA Date: Aug 2020 Ambergris, an excretion product of sperm whales, has been a valued agent in the formulation of perfumes. The composition of ambergris consists of two major components: 40–46% cholestanol type steroids and approximately 25–45% of a triterpenoid ... Read more
Application of biocatalysis in synthetic chemistry

Author: Brady, D ; Molefe, M ; Steenkamp, Lucia H ; Parkinson, C ; Jordaan, J ; Visser, Daniel F ; Simpson, C ; Chhiba, Varsha P ; Mathiba, K Date: Nov 2006 It was found that the nitrile hydratases in microorganisms isolated from around the world had infra-species amino acid sequence differences, and this provided an explanation for the variability of nitrile substrate usage within the species. ... Read more
Bacteria and yeast isolation and characterisation from a South African fermented beverage

Author: Moodley, Sanchia S ; Dlamini, Nomusa R ; Steenkamp, Lucia H ; Buys, Elna M Date: Nov 2019 Spontaneous fermentation of motoho, a southern African non-alcoholic sorghum beverage, results in products with inconsistent microbiological and sensory quality. We aimed to identify the microorganisms involved in the fermentation of motoho ... Read more
Biocatalytic enantiomeric resolution of l-menthol from an eight isomeric menthol mixture

Author: Brady, D ; Reddy, S ; Mboniswa, B ; Steenkamp, Lucia H ; Rousseau, A ; Parkinson, CJ ; Chaplin, J ; Mitra, RK ; Moutlana, T ; Marais, SF ; Gardiner, NS Date: Mar 2012 The four diastereomers of menthol and their enantiomers, namely dl-menthol, dl-neomenthol, dl-neoisomenthol and dl isomenthol, were synthesised by the hydrogenation of thymol to yield an eight isomer liquid menthol. A suitably selective ... Read more
Decision making in the development of a biocatalytic route for resolution of S-naproxen: from screening to scale-up

Author: Steenkamp, Lucia H ; Brady, D Date: Jun 2010 The non-steroidal anti-inflammatory drug naproxen is most effective as the single S-naproxen enantiomer. However, typical synthetic routes to naproxen yield the racemate of both the R and the S stereoisomers. Biocatalysts can be used to resolve ... Read more
Development of a two-step “green” synthesis for (-)-ambafuran production

Author: Steenkamp, Lucia H Date: 01 Sep 2010 The main objective of the development of a two-step “green” synthesis for (-)-ambafuran production is to find an alternative synthesis of (-) Ambrox from sclareol, to use a bioconversion or biocatalysis route, and that it results in a natural ... Read more
Gas chromatographic profiling of the biocatalytic conversion of sclareol to ambradiol by Hyphozyma roseoniger

Author: Ncube, EN ; Mathiba, Kgama ; Steenkamp, Lucia H ; Dubery, IA Date: Oct 2021 Hyphozyma roseoniger is a filamentous yeast used for its biocatalytic ability to convert sclareol, a plant diterpenoid to ambradiol, an intermediate to ambrafuran, which is a sought-after fixative in the perfume industry. Metabolite profiling ... Read more
Naproxen: from skunk work to scale-up

Author: Steenkamp, Lucia H ; Brady, D Date: Nov 2008 The presentation starts with a comparison between Naproxen and Aspirin. The characteristics and properties of Naproxen are explained, as well as the biocatalysis and the screening of the enzyme activity and selectivity. It ends with a discussion ... Read more
Optimisation of stabilised carboxylesterase NP for enantioselective hydrolysis of naproxen methyl ester

Author: Steenkamp, Lucia H ; Brady, D Date: Dec 2008 Although the enantioselective kinetic resolution of ester racemates of the non-steroidal antiinflammatory drug naproxen ([2-(6-methoxy-2-naphthyl) propionic acid]) is a common demonstration for biocatalysis, the enantiomeric excess of the ... Read more
Optimisation of the enantioselective biocatalytic hydrolysis of naproxen ethyl ester using ChiroCLEC-CR

Author: Brady, D ; Steenkamp, Lucia H ; Skein, E ; Chaplin, JA ; Reddy, S Date: 04 Mar 2004 In a biocatalytic reaction the immobilized lipase ChiroCLEC-CR enantioselectively hydrolysed a naproxen ethyl ester racemate, yielding (S)-naproxen with an enantiomeric excess of more than 98%, an enantiomeric ratio (E) of more than 100, and ... Read more
Profiling of botanical extracts for authentication, detection of adulteration and quality control using UPLC-QTOF-MS

Author: Steenkamp, Paul A ; Steenkamp, Lucia H ; Mancama, Dalubuhle T Date: Jan 2018 Herbs and plant extracts are very popular and are being used by all cultures for healthcare and as food supplements. The correct identification of the required plant material is vital for formulation of final formulas for use by mankind. The ... Read more
Real time PCR of Nor~1 (aflD) gene of aflatoxin producing fungi and its correlative quantization to aflatoxin levels in South African compound feeds

Author: Iheanacho, HE ; Dutton, MF ; Steenkamp, PA ; Steenkamp, Lucia H ; Makund, HA ; Swart, A ; Mthombeni, JQ Date: Feb 2014 Aflatoxins (AFs) are naturally occurring secondary metabolites. This toxin is principally produced by Aspergillus flavus and Aspergillus parasiticus in compound feeds worldwide. Compound feeds are feeds blended from various raw materials and ... Read more
Screening of commercial enzymes for the enantioselective hydrolysis of R,S-naproxen ester

Author: Steenkamp, Lucia H ; Brady, D Date: 03 Mar 2003 This study focused on the identification of suitable lipase or esterase activity for enantiomeric resolution of (R, S)-naproxen. For an economically viable reaction the enantiomeric ratio (E) should preferably be >100, while maximising the ... Read more
UPLC-MS profiling, identification of major peaks and comparison of Harpagophytum procumbens extracts from different locations

Author: Steenkamp, Paul A ; Steenkamp, Lucia H Date: Aug 2019 Organic extracts (methanol:acetonitrile (1:1 v/v)) of the dried and grounded secondary tubers of Devil's Claw, Harpagophytum procumbens (Burch.) DC. ex Meisn., produced extracts rich in iridoid glucosides. An UPLC-MS profiling method was ... Read more

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