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Biolistic mediated sorghum (Sorghum bicolor L. Moench) transformation via mannose and bialaphos based selection systems

Author: Grootboom, AW ; Mkhonza, NL ; O'Kennedy, Maretha M ; Chakauya, E ; Kunert, K ; Chikwamba, Rachel K Date: 2010 Sorghum (Sorghum bicolor (L.) Moench) is recalcitrant to transformation and besides several reported cases of success, transformation is far from routine. The choice of the selectable markers is key factor influencing success and efficiency ... Read more
Co-suppression of synthesis of major x-kafirin sub-class together with y-kafirin-1 and y-kafirin-2 required for substantially improved protein digestibility in transgenic sorghum

Author: Grootboom, AW ; Mkhonza, NL ; Mbambo, Z ; O'Kennedy, Maretha M ; Da Silva, LS ; Taylor, J ; Taylor, JRN ; Chikwamba, Rachel K ; Mehlo, L Date: Jan 2014 Co-suppressing major kafirin sub-classes is fundamental to improved protein digestibility and nutritional value of sorghum. The improvement is linked to an irregularly invaginated phenotype of protein bodies. Read more
Condensed tannins in traditional wet-cooked and modern extrusion-cooked sorghum porridges

Author: Dlamini, Nomusa R ; Dykes, L ; Rooney, LW ; Waniska, RD ; Taylor, JRN Date: Jul 2009 The profile and quantities of condensed tannins (CT) in foods are affected by processing due to their highly reactive nature, which may affect their antioxidant activity and the nutritional value of the foods. The objective was to compare the ... Read more
Detecting changes in the nutritional value and elemental composition of transgenic sorghum grain

Author: Ndimba, R ; Grootboom, AW ; Mehlo, L ; Mkhonza, NL ; Kossmann, J ; Barnabas, AD ; Mtshali, C ; Pineda-Vargasa, C Date: Sep 2015 We have previously demonstrated that poor digestibility in sorghum can be addressed by using RNA interference (RNAi) to suppress kafirin synthesis. The approach resulted in a twofold improvement in overall protein digestibility levels. In the ... Read more
Development of a protein-rich composite sorghum-cowpea instant porridge by extrusion cooking process

Author: Pelembe, LAM ; Erasmus, C ; Taylor, JRN Date: 2002 To develop instant high protein porridge, various ratios of sorghum and cowpeas were extruded at 130 and 165 degrees C and a water content of 200 g/kg using a thin-screw extruder. An increased proportion of cowpeas resulted in an increase in ... Read more
Effect of processing on β-carotene levels in sorghum

Author: Reddy, J ; Erasmus, C ; Mlotshwa, L ; Taylor, J ; Dlamini, Nomusa R Date: 01 Sep 2010 Sorghum, a staple food in Africa, does not contain adequate amounts of provitamin A carotenoids to address the problem of vitamin A deficiency which affects up to 31 million people on the continent1. One attempt to solve this problem is through ... Read more
Harnessing sorghum and millet biotechnology for food and health

Author: O'Kennedy, Maretha M ; Grootboom, A ; Shewry, PR Date: Nov 2006 This review highlights recombinant DNA technology as a powerful tool to enhance the gene pools of sorghum and pearl millet crops regarded as jewels of Africa. Although important advances in the improvement of these species have been made by ... Read more
Induced protein polymorphisms and nutritional quality of gamma irradiation mutants of sorghum

Author: Mehlo, L ; Mbambo, Z ; Bado, S ; Lin, J ; Moagi, SM ; Buthelezi, Sindisiwe G ; Stoychev, Stoyan H ; Chikwamba, Rachel K Date: Sep 2013 Physical and biochemical analysis of protein polymorphisms in seed storage proteins of a mutant population of sorghum revealed a mutant with redirected accumulation of kafirin proteins in the germ. The change in storage proteins was accompanied ... Read more
Production, consumption, and quality attributes of Akpan – a yoghurt-like cereal product from West Africa

Author: Sassa, C ; Adinsi, L ; Anihouvi, V ; Akissoe, N ; Dalode, G ; Mestres, C ; Jacobs, A ; Dlamini, N ; Pallet, D ; Hounhouigan, D Date: May 2012 Akpan is a high-potential, traditional yoghurt-like product made from fermented cereal starch, and consumed as a thirst-quenching beverage in Benin. This study investigated the characteristics of consumers, the traditional processing techniques ... Read more
Proton induced X-ray emission and electron microscopy analysis of induced mutants of sorghum

Author: Mbambo, Z ; Minnis-Ndimba, R ; Pineda, C ; Ndimba, B ; Bado, S ; Lin, J ; Chikwamba, Rachel K ; Mehlo, L Date: 2014 Gamma irradiation induced alterations in the spatial distribution and localization profiles of Fe, Zn, K, P, Cl, Ca, S, Mn, and Cu in mutant seeds of sorghum were determined using PIXE. The changes included enhanced or diminished accumulation ... Read more
Transcriptomic analysis of a Sorghum bicolor landrace identifies a role for beta-alanine betaine biosynthesis in drought tolerance

Author: Devnarain, A ; Crampton, BG ; Olivier, N ; Van der Westhuyzen, Christiaan W ; Becker, JVW ; O'Kennedy, Maretha M Date: Dec 2019 Sorghum is indigenous to Africa and a remarkably drought tolerant cereal crop. In this study, the genetic response mechanisms involved in sorghum's tolerance to progressive water deficit and re-watering were investigated in a South African ... Read more
Using ICP and micro-PIXE to investigate possible differences in the mineral composition of genetically modified versus wild-type sorghum grain

Author: Ndimba, R ; Cloete, K ; Mehlo, Luke ; Kossmann, J ; Mtshali, C ; Pineda-Vargas, C Date: Aug 2017 In the present study, possible differences in the mineral composition of transgenic versus non-transgenic sorghum grains were investigated using inductively coupled atomic emission spectroscopy (ICP-AES); and, in-tissue elemental mapping by ... Read more

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Browsing by Subject "Sorghum"

Browsing by Subject "Sorghum"

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